ArticleProceedings of the National Academy of Sciences of the United States of America2002
Adenovirus-mediated gene delivery and in vitro microinsemination produce offspring from infertile male mice.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 78 citations in OpenAlex.
- Messenger RNA delivery into Sertoli cells restores fertility to congenitally infertile male mice.Stem cell reports · 2026Article
- In vitro system completely restores oogenesis in congenitally infertile mice.Human reproduction (Oxford, England) · 2025Article
- Sperm and offspring production in a nonobstructive azoospermia mouse model via testicular mRNA delivery using lipid nanoparticles.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Sendai virus-mediated RNA delivery restores fertility to congenital and chemotherapy-induced infertile female mice.PNAS nexus · 2024Article
- Adenovirus-mediated gene delivery restores fertility in congenitally infertile female mice.The Journal of reproduction and development · 2022Article
- Sertoli cell ablation and replacement of the spermatogonial niche in mouse.Nature communications · 2020Article
- In Vivo Genetic Manipulation of Spermatogonial Stem Cells and Their Microenvironment by Adeno-Associated Viruses.Stem cell reports · 2018Article
- Probing GATA factor function in mouse Leydig cells via testicular injection of adenoviral vectors.Reproduction (Cambridge, England) · 2017Article
- Experimental methods to preserve male fertility and treat male factor infertility.Fertility and sterility · 2016Review
- Mouse Spermatogenesis Requires Classical and Nonclassical Testosterone Signaling.Biology of reproduction · 2016Article
- Sustained expression of insulin by a genetically engineered sertoli cell line after allotransplantation in diabetic BALB/c mice.Biology of reproduction · 2014Article
- Nondividing, postpubertal rat sertoli cells resumed proliferation after transplantation.Biology of reproduction · 2014Article
- Testis tissue explantation cures spermatogenic failure in c-Kit ligand mutant mice.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- In vitro production of fertile sperm from murine spermatogonial stem cell lines.Nature communications · 2011Article
- Genomic integration of adenoviral gene transfer vectors following transduction of fertilized mouse oocytes.Transgenic research · 2011Article
- Lentiviral mediated transgenesis by in vivo manipulation of spermatogonial stem cells.PloS one · 2011Article
- Spermatogonial stem cells derived from infertile Wv/Wv mice self-renew in vitro and generate progeny following transplantation.Biology of reproduction · 2009Article
- Intra-testicular injection of adenoviral constructs results in Sertoli cell-specific gene expression and disruption of the seminiferous epithelium.Reproduction (Cambridge, England) · 2009Article
- Adenovirus-mediated gene delivery into mouse spermatogonial stem cells.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Propagation of an infertile hermaphrodite mouse lacking germ cells by using nuclear transfer and embryonic stem cell technology.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sertoli cells play a pivotal role in spermatogenesis through their interactions with germ cells. To set up a strategy for treating male infertility caused by Sertoli cell dysfunction, we developed a Sertoli cell gene transfer system by using an adenovirus vector, which maintained long-term transgene expression in the testes of infertile mice. Introduction of an adenovirus carrying the mouse Steel (Sl) gene into Sertoli cells restored partial spermatogenesis in infertile Steel/Steel(dickie) (Sl/Sl(d)) mutant mouse testes. Although these males remained infertile, round spermatids and spermatozoa from the testes produced normal fertile offspring after intracytoplasmic injection into oocytes. None of the offspring showed evidence of germ line transmission of adenoviral DNA. Thus, we demonstrate a successful treatment for infertility by using a gene therapy vector. Therefore, adenovirus-mediated gene delivery into Sertoli cells not only provides an efficient and convenient means for studying germ cell-Sertoli cell interactions through manipulation of the germ cell microenvironment in vivo, but also is a useful method to treat male infertility resulting from a Sertoli cell defect.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.