ArticleProceedings of the National Academy of Sciences of the United States of America2002
A cell number-counting factor regulates the cytoskeleton and cell motility in Dictyostelium.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 48 citations in OpenAlex.
- Transcriptomics informed discovery of developmentally essential transcription factors.Biology open · 2025Article
- An altered transcriptome underliesFrontiers in genetics · 2022Article
- A telomerase with novel non-canonical roles: TERT controls cellular aggregation and tissue size in Dictyostelium.PLoS genetics · 2019Article
- An endogenous chemorepellent directs cell movement by inhibiting pseudopods at one side of cells.Molecular biology of the cell · 2019Article
- Different Isoforms of the Neuronal Guidance Molecule Slit2 Directly Cause Chemoattraction or Chemorepulsion of Human Neutrophils.Journal of immunology (Baltimore, Md. : 1950) · 2019Article
- The Use of Diffusion Calculations and Monte Carlo Simulations to Understand the Behavior of Cells inComputational and structural biotechnology journal · 2019Review
- Article
- Collective regulation of cell motility using an accurate density-sensing system.Journal of the Royal Society, Interface · 2018Article
- Protease activated-receptor 2 is necessary for neutrophil chemorepulsion induced by trypsin, tryptase, or dipeptidyl peptidase IV.Journal of leukocyte biology · 2018Article
- Response of Dictyostelium discoideum to UV-C and involvement of poly (ADP-ribose) polymerase.Cell proliferation · 2015Article
- Cell signaling during development of Dictyostelium.Developmental biology · 2014Review
- Copine A is expressed in prestalk cells and regulates slug phototaxis and thermotaxis in developing Dictyostelium.Development, growth & differentiation · 2011Article
- Cell density sensing and size determination.Development, growth & differentiation · 2011Review
- A quorum-sensing factor in vegetative Dictyostelium discoideum cells revealed by quantitative migration analysis.PloS one · 2011Article
- A cell number counting factor alters cell metabolism.Communicative & integrative biology · 2009Article
- Dictyostelium cells bind a secreted autocrine factor that represses cell proliferation.BMC biochemistry · 2009Article
- A protein with similarity to PTEN regulates aggregation territory size by decreasing cyclic AMP pulse size during Dictyostelium discoideum development.Eukaryotic cell · 2008Article
- Combining experiments and modelling to understand size regulation in Dictyostelium discoideum.Journal of the Royal Society, Interface · 2008Review
- The secreted Dictyostelium protein CfaD is a chalone.Journal of cell science · 2008Article
- SrfB, a member of the Serum Response Factor family of transcription factors, regulates starvation response and early development in Dictyostelium.Developmental biology · 2008Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Little is known about how a morphogenetic rearrangement of a tissue is affected by individual cells. Starving Dictyostelium discoideum cells aggregate to form dendritic streams, which then break up into groups of approximately 2 x 10(4) cells. Cell number is sensed at this developmental stage by using counting factor (CF), a secreted complex of polypeptides. A high extracellular concentration of CF indicates that there is a large number of cells, which then causes the aggregation stream to break up. Computer simulations indicated that stream breakup could be caused by CF decreasing cell-cell adhesion and/or increasing cell motility, and we observed that CF does indeed decrease cell-cell adhesion. We find here that CF increases cell motility. In Dictyostelium, motility is mediated by actin and myosin. CF increases the amounts of polymerized actin and the ABP-120 actin-crosslinking protein. Partially inhibiting motility by using drugs that interfere with actin polymerization reduces stream dissipation, resulting in fewer stream breaks and thus larger groups. CF also potentiates the phosphorylation and redistribution of myosin while repressing its basal level of assembly. The computer simulations indicated that a narrower distribution of group sizes results when a secreted factor modulates both adhesion and motility. CF thus seems to induce the morphogenesis of streams into evenly sized groups by increasing actin polymerization, ABP-120 levels, and myosin phosphorylation and decreasing adhesion and myosin polymerization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.