ArticleThe Journal of general physiology2002
Conductance and permeability of the residual state of connexin43 gap junction channels.
Article in The Journal of general physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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48 citing papers in PubMed, 83 citations in OpenAlex.
- Nernst equilibrium, rectification, and saturation: Insights into ion channel behavior.Biophysical journal · 2024Article
- Nernst Equilibrium, Rectification, and Saturation: Insights into Ion Channel Behavior.bioRxiv : the preprint server for biology · 2024Article
- Connexin Gap Junction Channels and Hemichannels: Insights from High-Resolution Structures.Biology · 2024Review
- Non-trivial dynamics in a model of glial membrane voltage driven by open potassium pores.Biophysical journal · 2023Article
- Function and Plasticity of Electrical Synapses in the Mammalian Brain: Role of Non-Junctional Mechanisms.Biology · 2022Review
- Connexins in the Heart: Regulation, Function and Involvement in Cardiac Disease.International journal of molecular sciences · 2021Review
- Four-State Model for Simulating Kinetic and Steady-State Voltage-Dependent Gating of Gap Junctions.Biophysical journal · 2020Article
- Gap19, a Cx43 Hemichannel Inhibitor, Acts as a Gating Modifier That Decreases Main State Opening While Increasing Substate Gating.International journal of molecular sciences · 2020Article
- Structure and Function of Cochlear Gap Junctions and Implications for the Translation of Cochlear Gene Therapies.Frontiers in cellular neuroscience · 2019Article
- Electrical coupling and its channels.The Journal of general physiology · 2018Review
- Lipidated connexin mimetic peptides potently inhibit gap junction-mediated CaAmerican journal of physiology. Cell physiology · 2018Article
- Gating of Connexin Channels by transjunctional-voltage: Conformations and models of open and closed states.Biochimica et biophysica acta. Biomembranes · 2018Review
- Permeant-specific gating of connexin 30 hemichannels.The Journal of biological chemistry · 2017Article
- 3D time-varying simulations of CaInternational journal for numerical methods in biomedical engineering · 2017Article
- Stochastic Model of Gap Junctions Exhibiting Rectification and Multiple Closed States of Slow Gates.Biophysical journal · 2016Article
- Reverberation of excitation in neuronal networks interconnected through voltage-gated gap junction channels.The Journal of general physiology · 2016Article
- Voltage regulation of connexin channel conductance.Yonsei medical journal · 2015Review
- Investigation of connexin 43 uncoupling and prolongation of the cardiac QRS complex in preclinical and marketed drugs.British journal of pharmacology · 2014Article
- Pannexins form gap junctions with electrophysiological and pharmacological properties distinct from connexins.Scientific reports · 2014Article
- Hyperthermia differently affects connexin43 expression and gap junction permeability in skeletal myoblasts and HeLa cells.Mediators of inflammation · 2014Article
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3 authors at 1 institution in 1 country.
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Abstract
We used cell lines expressing wild-type connexin43 and connexin43 fused with the enhanced green fluorescent protein (Cx43-EGFP) to examine conductance and perm-selectivity of the residual state of Cx43 homotypic and Cx43/Cx43-EGFP heterotypic gap junction channels. Each hemichannel in Cx43 cell-cell channel possesses two gates: a fast gate that closes channels to the residual state and a slow gate that fully closes channels; the transjunctional voltage (V(j)) closes the fast gate in the hemichannel that is on the relatively negative side. Here, we demonstrate macroscopically and at the single-channel level that the I-V relationship of the residual state rectifies, exhibiting higher conductance at higher V(j)s that are negative on the side of gated hemichannel. The degree of rectification increases when Cl(-) is replaced by Asp(-) and decreases when K(+) is replaced by TEA(+). These data are consistent with an increased anionic selectivity of the residual state. The V(j)-gated channel is not permeable to monovalent positively and negatively charged dyes, which are readily permeable through the fully open channel. These data indicate that a narrowing of the channel pore accompanies gating to the residual state. We suggest that the fast gate operates through a conformational change that introduces positive charge at the cytoplasmic vestibule of the gated hemichannel, thereby producing current rectification, increased anionic selectivity, and a narrowing of channel pore that is largely responsible for reducing channel conductance and restricting dye transfer. Consequently, the fast V(j)-sensitive gating mechanism can serve as a selectivity filter, which allows electrical coupling but limits metabolic communication.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.