ArticleBritish journal of pharmacology2002
A study of P2X1 receptor function in murine megakaryocytes and human platelets reveals synergy with P2Y receptors.
Article in British journal of pharmacology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 77 citations in OpenAlex.
- Exploring bias in platelet P2YBritish journal of pharmacology · 2024Review
- The Impact of Cold Storage on Adenosine Diphosphate-Mediated Platelet Responsiveness.TH open : companion journal to thrombosis and haemostasis · 2020Article
- Development of a P2X1-eYFP receptor knock-in mouse to track receptors in real time.Purinergic signalling · 2019Article
- Article
- Expression and function of purinergic receptors in platelets from apheresis-derived platelet concentrates.Blood transfusion = Trasfusione del sangue · 2016Article
- Purinergic signaling and infection by Leishmania: A new approach to evasion of the immune response.Biomedical journal · 2016Review
- Role of Purinergic Receptor Expression and Function for Reduced Responsiveness to Adenosine Diphosphate in Washed Human Platelets.PloS one · 2016Article
- Blood cells: an historical account of the roles of purinergic signalling.Purinergic signalling · 2015Review
- Purinergic control of inflammation and thrombosis: Role of P2X1 receptors.Computational and structural biotechnology journal · 2015Review
- Subtype-specific control of P2X receptor channel signaling by ATP and Mg2+.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Using antibodies against P2Y and P2X receptors in purinergic signaling research.Purinergic signalling · 2012Article
- The P2X1 receptor and platelet function.Purinergic signalling · 2011Article
- Acetylsalicylic acid enhances purinergic receptor-mediated outward currents in rat megakaryocytes.American journal of physiology. Cell physiology · 2010Article
- Article
- Functional evidence for the expression of P2X1, P2X4 and P2X7 receptors in human lung mast cells.British journal of pharmacology · 2009Article
- Interaction of P2 purinergic receptors with cellular macromolecules.Naunyn-Schmiedeberg's archives of pharmacology · 2008Review
- Primary and secondary agonists can use P2X(1) receptors as a major pathway to increase intracellular Ca(2+) in the human platelet.Journal of thrombosis and haemostasis : JTH · 2007Article
- International Union of Pharmacology LVIII: update on the P2Y G protein-coupled nucleotide receptors: from molecular mechanisms and pathophysiology to therapy.Pharmacological reviews · 2006Review
- P2 receptors: intracellular signaling.Pflugers Archiv : European journal of physiology · 2006Review
- Disruption of lipid rafts inhibits P2X1 receptor-mediated currents and arterial vasoconstriction.The Journal of biological chemistry · 2005Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have examined the role of ATP-dependent P2X(1) receptors in megakaryocytes (MKs) and platelets using receptor-deficient mice and selective agonists. Alpha,beta-meATP- and ATP- evoked ionotropic inward currents were absent in whole-cell recordings from MKs of P2X(1)(-/-) mice, demonstrating that the P2X receptor phenotype in MKs, and by inference, platelets, is due to expression of homomeric P2X(1) receptors. P2X(1) receptor deficiency had no effect on MK (CD 41) numbers or size distribution, showing that it is not essential for normal MK development. P2Y receptor-stimulated [Ca(2+)](i) responses were unaffected in MKs from P2X(1)(-/-) mice, however the inward cation current associated with Ca(2+) release was reduced by approximately 50%, suggesting an interaction between the membrane conductances activated by P2X(1) and P2Y receptors. Interaction between P2X(1) and P2Y receptors in human platelets was also examined using [Ca(2+)](i) recordings from cell suspensions. Alpha,beta-meATP (10 microM) evoked a rapid transient P2X(1) receptor-mediated increase in [Ca(2+)](i), whereas ADP-(10 microM) evoked P2Y receptor responses were slower, peaked at a higher level and remained elevated for longer periods. Co-application of alpha, beta-meATP and ADP resulted in marked acceleration and amplification of the peak [Ca(2+)](i) response. We conclude that ionotropic P2X(1) receptors may play a priming role in the subsequent activation of metabotropic P2Y receptors during platelet stimulation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.