ArticleNucleic acids research2001
The trypanosome homolog of human p32 interacts with RBP16 and stimulates its gRNA binding activity.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 42 citations in OpenAlex.
- DRBD3 regulates long non-coding RNA abundance and cryptic splice site selection in trypanosomes.Cellular and molecular life sciences : CMLS · 2025Article
- DRBD18 acts as a transcript-specific RNA editing auxiliary factor inRNA (New York, N.Y.) · 2025Article
- A role for a Trypanosoma brucei cytosine RNA methyltransferase homolog in ribosomal RNA processing.PloS one · 2024Article
- Translational control byRNA (New York, N.Y.) · 2023Article
- Selective nuclear export of mRNAs is promoted by DRBD18 in Trypanosoma brucei.Molecular microbiology · 2021Article
- Trypanosome RNAEditing Substrate Binding Complex integrity and function depends on the upstream action of RESC10.Nucleic acids research · 2021Article
- Divergent Small Tim Homologues Are Associated with TbTim17 and Critical for the Biogenesis of TbTim17 Protein Complexes inmSphere · 2018Article
- Y-box protein-associated acidic protein (YBAP1/C1QBP) affects the localization and cytoplasmic functions of YB-1.Scientific reports · 2018Article
- MRB7260 is essential for productive protein-RNA interactions within the RNA editing substrate binding complex during trypanosome RNA editing.RNA (New York, N.Y.) · 2018Article
- The Major Protein Arginine Methyltransferase inThe Journal of biological chemistry · 2017Article
- Interactions between RNA-binding proteins and P32 homologues in trypanosomes and human cells.Current genetics · 2016Article
- Arginine methylation of DRBD18 differentially impacts its opposing effects on the trypanosome transcriptome.Nucleic acids research · 2015Article
- An arginine-glycine-rich RNA binding protein impacts the abundance of specific mRNAs in the mitochondria of Trypanosoma brucei.Eukaryotic cell · 2015Article
- Functional interplay between protein arginine methyltransferases in Trypanosoma brucei.MicrobiologyOpen · 2014Article
- Identification of a novel lipin homologue from the parasitic protozoan Trypanosoma brucei.BMC microbiology · 2013Article
- Endonuclease associations with three distinct editosomes in Trypanosoma brucei.The Journal of biological chemistry · 2011Article
- MRB3010 is a core component of the MRB1 complex that facilitates an early step of the kinetoplastid RNA editing process.RNA (New York, N.Y.) · 2011Article
- TbRGG2 facilitates kinetoplastid RNA editing initiation and progression past intrinsic pause sites.RNA (New York, N.Y.) · 2010Article
- Structure of the Trypanosoma brucei p22 protein, a cytochrome oxidase subunit II-specific RNA-editing accessory factor.The Journal of biological chemistry · 2010Article
- gRNA/pre-mRNA annealing and RNA chaperone activities of RBP16.RNA (New York, N.Y.) · 2008Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
RBP16 is a guide RNA (gRNA)-binding protein that was shown through immunoprecipitation experiments to interact with approximately 30% of total gRNAs in Trypanosoma brucei mitochondria. To gain insight into the biochemical function of RBP16, we used affinity chromatography and immunoprecipitation to identify RBP16 protein binding partners. By these methods, RBP16 does not appear to stably interact with the core editing machinery. However, fractionation of mitochondrial extracts on MBP-RBP16 affinity columns consistently isolated proteins of 12, 16, 18 and 22 kDa that were absent from MBP control columns. We describe here our analysis of one RBP16-associated protein, p22. The predicted p22 protein has significant sequence similarity to a family of multimeric, acidic proteins that includes human p32 and Saccharomyces cerevisiae mam33p. Glutaraldehyde crosslinking of recombinant p22 identified homo-multimeric forms of the protein, further substantiating its homology to p32. We confirmed the p22-RBP16 interaction and demonstrated that the two proteins bind each other directly by ELISA utilizing recombinant p22 and RBP16. p32 family members have been reported to modulate viral and cellular pre-mRNA splicing, in some cases by perturbing interaction of their binding partners with RNA. To determine whether p22 similarly affects the gRNA binding properties of RBP16, we titrated recombinant p22 into UV crosslinking assays. These experiments revealed that p22 significantly stimulates the gRNA binding capacity of RBP16. Thus, p22 has the potential to be a regulatory factor in T.brucei mitochondrial gene expression by modulating the RNA binding properties of RBP16.
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