ArticleNucleic acids research2002
A Pyrococcus homolog of the leucine-responsive regulatory protein, LrpA, inhibits transcription by abrogating RNA polymerase recruitment.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 59 citations in OpenAlex.
- CopR, a Global Regulator of Transcription to Maintain Copper Homeostasis inFrontiers in microbiology · 2020Article
- Dissecting the Repertoire of DNA-Binding Transcription Factors of the Archaeon Pyrococcus furiosus DSM 3638.Life (Basel, Switzerland) · 2018Article
- The Transcriptional Regulator TFB-RF1 Activates Transcription of a Putative ABC Transporter inFrontiers in microbiology · 2018Article
- Structure and mechanisms of viral transcription factors in archaea.Extremophiles : life under extreme conditions · 2017Review
- A conserved hexanucleotide motif is important in UV-inducible promoters in Sulfolobus acidocaldarius.Microbiology (Reading, England) · 2017Article
- The interplay between nucleoid organization and transcription in archaeal genomes.Nature reviews. Microbiology · 2015Review
- Archaeal RNA polymerase and transcription regulation.Critical reviews in biochemistry and molecular biology · 2011Review
- The Lrp family of transcription regulators in archaea.Archaea (Vancouver, B.C.) · 2010Article
- Transcriptional control by two leucine-responsive regulatory proteins in Halobacterium salinarum R1.BMC molecular biology · 2010Article
- The DNA-recognition mode shared by archaeal feast/famine-regulatory proteins revealed by the DNA-binding specificities of TvFL3, FL10, FL11 and Ss-LrpB.Nucleic acids research · 2009Article
- SurR: a transcriptional activator and repressor controlling hydrogen and elemental sulphur metabolism in Pyrococcus furiosus.Molecular microbiology · 2009Article
- Crystal structure of glutamine receptor protein from Sulfolobus tokodaii strain 7 in complex with its effector L-glutamine: implications of effector binding in molecular association and DNA binding.Nucleic acids research · 2008Article
- Characterization of the transcriptional activity of the cryptic plasmid pRN1 from Sulfolobus islandicus REN1H1 and regulation of its replication operon.Journal of bacteriology · 2007Article
- A novel archaeal regulatory protein, Sta1, activates transcription from viral promoters.Nucleic acids research · 2006Article
- An expanding family of archaeal transcriptional activators.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Regulation of tryptophan operon expression in the archaeon Methanothermobacter thermautotrophicus.Journal of bacteriology · 2005Article
- The role of cis-acting sequences governing catabolite repression control of lacS expression in the archaeon Sulfolobus solfataricus.Genetics · 2004Article
- Revealing gene transcription and translation initiation patterns in archaea, using an interactive clustering model.Extremophiles : life under extreme conditions · 2004Article
- Occurrence and characterization of mercury resistance in the hyperthermophilic archaeon Sulfolobus solfataricus by use of gene disruption.Journal of bacteriology · 2004Article
- Activation of archaeal transcription by recruitment of the TATA-binding protein.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The genomes of Archaea harbor homologs of the global bacterial regulator leucine-responsive regulatory protein (Lrp). Archaeal Lrp homologs are helix-turn-helix DNA-binding proteins that specifically repress the transcription of their own genes in vitro. Here, we analyze the interaction of Pyrococcus LrpA with components of the archaeal transcriptional machinery at the lrpA promoter. DNA-protein complexes can be isolated by electrophoretic mobility shift assays that contain both LrpA and the two archaeal transcription factors TBP and TFB. Phenanthroline-copper footprinting experiments showed that the DNA-binding sites of LrpA and TBP/TFB do not overlap. These results and the finding that association of RNA polymerase with the TBP-TFB promoter complex was inhibited in the presence of LrpA indicate that Pyrococcus LrpA interferes with RNA polymerase recruitment. A DNA motif required for the LrpA-DNA interaction was inferred from dimethylsulfate methylation interference experiments.
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