ArticleNucleic acids research2002
Phosphorylation of Rph1, a damage-responsive repressor of PHR1 in Saccharomyces cerevisiae, is dependent upon Rad53 kinase.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 31 citations in OpenAlex.
- Histone Demethylase MoRph1 Regulates Fungal Development, Pathogenicity, and DNA Damage Repair inJournal of fungi (Basel, Switzerland) · 2026Article
- Article
- An optogenetic switch for the Set2 methyltransferase provides evidence for transcription-dependent and -independent dynamics of H3K36 methylation.Genome research · 2020Article
- Checkpoint Responses to DNA Double-Strand Breaks.Annual review of biochemistry · 2020Review
- Rpd3L Contributes to the DNA Damage Sensitivity ofGenetics · 2019Article
- A pathway of targeted autophagy is induced by DNA damage in budding yeast.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Live longer on MARS: a yeast paradigm of mitochondrial adaptive ROS signaling in aging.Microbial cell (Graz, Austria) · 2014Review
- Dissociation of the H3K36 demethylase Rph1 from chromatin mediates derepression of environmental stress-response genes under genotoxic stress in Saccharomyces cerevisiae.Molecular biology of the cell · 2013Article
- Checkpoint kinases regulate a global network of transcription factors in response to DNA damage.Cell reports · 2013Article
- Epigenetic silencing mediates mitochondria stress-induced longevity.Cell metabolism · 2013Article
- Cbk1 kinase and Bck2 control MAP kinase activation and inactivation during heat shock.Molecular biology of the cell · 2011Article
- The histone H3K36 demethylase Rph1/KDM4 regulates the expression of the photoreactivation gene PHR1.Nucleic acids research · 2011Article
- Life in the midst of scarcity: adaptations to nutrient availability in Saccharomyces cerevisiae.Current genetics · 2010Review
- Perspectives on the DNA damage and replication checkpoint responses in Saccharomyces cerevisiae.DNA repair · 2009Review
- Characterization of the rapamycin-sensitive phosphoproteome reveals that Sch9 is a central coordinator of protein synthesis.Genes & development · 2009Article
- Pdr3 is required for DNA damage induction of MAG1 and DDI1 via a bi-directional promoter element.Nucleic acids research · 2004Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rph1, a Cys2-His2 zinc finger protein, binds to an upstream repressing sequence of the photolyase gene PHR1, and represses its transcription in response to DNA damage in Saccharomyces cerevisiae. In this report, we have demonstrated that the phosphorylation of Rph1 protein was increased in response to DNA damage. The DNA damage-induced phosphorylation of Rph1 was missing in most damage checkpoint mutants including rad9, rad17, mec1 and rad53. These results indicate that Rph1 phosphorylation is under the control of the Mec1-Rad53 damage checkpoint pathway. Rph1 phosphorylation required the kinase activity of Rad53 since it was significantly decreased in rad53 checkpoint mutant. Furthermore, loss of other kinases including Dun1, Tel1 and Chk1, which function downstream of Mec1, did not affect the Rph1 phosphorylation. This contrasts with the derepression of Crt1-regulated genes, which requires both Rad53 and Dun1 protein kinases. These results imply that post-translational modification of Rph1 repressor is regulated by a potentially novel damage checkpoint pathway that is distinct from the RAD53-DUN1-CRT1 cascade implicated in the DNA damage-dependent transcription of ribonucleotide reductase genes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.