ArticleThe Journal of cell biology2002
The integrin cytoplasmic domain-associated protein ICAP-1 binds and regulates Rho family GTPases during cell spreading.
Article in The Journal of cell biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 63 citations in OpenAlex.
- KRIT1: A Traffic Warden at the Busy Crossroads Between Redox Signaling and the Pathogenesis of Cerebral Cavernous Malformation Disease.Antioxidants & redox signaling · 2023Review
- Force tuning through regulation of clathrin-dependent integrin endocytosis.The Journal of cell biology · 2023Article
- CSK-mediated signalling by integrins in cancer.Frontiers in cell and developmental biology · 2023Review
- Review
- Serine phosphorylation of the small phosphoprotein ICAP1 inhibits its nuclear accumulation.The Journal of biological chemistry · 2020Article
- Modulation of small GTPase activity by NME proteins.Laboratory investigation; a journal of technical methods and pathology · 2018Review
- In vivo adhesion of malignant B cells to bone marrow microvasculature is regulated by α4β1 cytoplasmic-binding proteins.Leukemia · 2016Article
- Cell-Substrate Interactions Feedback to Direct Cell Migration along or against Morphological Polarization.PloS one · 2015Article
- Calcium and calmodulin-dependent serine/threonine protein kinase type II (CaMKII)-mediated intramolecular opening of integrin cytoplasmic domain-associated protein-1 (ICAP-1α) negatively regulates β1 integrins.The Journal of biological chemistry · 2013Article
- Concepts and hypothesis: integrin cytoplasmic domain-associated protein-1 (ICAP-1) as a potential player in cerebral cavernous malformation.Journal of neurology · 2013Review
- Ccm1 regulates microvascular morphogenesis during angiogenesis.Journal of vascular research · 2011Article
- p27kip1 controls cell morphology and motility by regulating microtubule-dependent lipid raft recycling.Molecular and cellular biology · 2010Article
- Role of nm23 in the regulation of cell shape and migration via Rho family GTPase signals.Molecular and cellular biochemistry · 2009Article
- KRIT-1/CCM1 is a Rap1 effector that regulates endothelial cell cell junctions.The Journal of cell biology · 2007Article
- Defective osteoblast function in ICAP-1-deficient mice.Development (Cambridge, England) · 2007Article
- In vivo dynamics of Rac-membrane interactions.Molecular biology of the cell · 2006Article
- Two distinct cytoplasmic regions of the beta2 integrin chain regulate RhoA function during phagocytosis.The Journal of cell biology · 2006Article
- Tissue-specific RNAi reveals that WT1 expression in nurse cells controls germ cell survival and spermatogenesis.Genes & development · 2006Article
- Genetics of cerebral cavernous malformations.Current neurology and neuroscience reports · 2005Review
- RhoGDI: multiple functions in the regulation of Rho family GTPase activities.The Biochemical journal · 2005Review
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using two-hybrid screening, we isolated the integrin cytoplasmic domain-associated protein (ICAP-1), an interactor for the COOH terminal region of the beta1A integrin cytoplasmic domain. To investigate the role of ICAP-1 in integrin-mediated adhesive function, we expressed the full-length molecule in NIH3T3 cells. ICAP-1 expression strongly prevents NIH3T3 cell spreading on extracellular matrix. This inhibition is transient and can be counteracted by coexpression of a constitutively activated mutant of Cdc42, suggesting that ICAP-1 acts upstream of this GTPase. In addition, we found that ICAP-1 binds both to Cdc42 and Rac1 in vitro, and its expression markedly inhibits activation of these GTPases during integrin-mediated cell adhesion to fibronectin as detected by PAK binding assay. In the attempt to define the molecular mechanism of this inhibition, we show that ICAP-1 reduces both the intrinsic and the exchange factor-induced dissociation of GDP from Cdc42; moreover, purified ICAP-1 displaces this GTPase from cellular membranes. Together, these data show for the first time that ICAP-1 regulates Rho family GTPases during integrin-mediated cell matrix adhesion, acting as guanine dissociation inhibitor.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.