ArticleBlood2002
Cyclin A transcriptional suppression is the major mechanism mediating homocysteine-induced endothelial cell growth inhibition.
Article in Blood, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 65 citations in OpenAlex.
- Nicotinamide N-Methyltransferase (NNMT): A New Hope for Treating Aging and Age-Related Conditions.Metabolites · 2024Review
- 1,6-Hexanediol regulates angiogenesis via suppression of cyclin A1-mediated endothelial function.BMC biology · 2023Article
- Identification of molecular subtypes of coronary artery disease based on ferroptosis- and necroptosis-related genes.Frontiers in genetics · 2022Article
- Endogenous hydrogen sulfide and ERK1/2-STAT3 signaling pathway may participate in the association between homocysteine and hypertension.Journal of geriatric cardiology : JGC · 2019Article
- Notoginsenoside Fc Accelerates Reendothelialization following Vascular Injury in Diabetic Rats by Promoting Endothelial Cell Autophagy.Journal of diabetes research · 2019Article
- Canagliflozin Inhibits Human Endothelial Cell Proliferation and Tube Formation.Frontiers in pharmacology · 2019Article
- Glutaminase-1 stimulates the proliferation, migration, and survival of human endothelial cells.Biochemical pharmacology · 2018Article
- Identification of homocysteine-suppressive mitochondrial ETC complex genes and tissue expression profile - Novel hypothesis establishment.Redox biology · 2018Article
- A computational approach to identify cellular heterogeneity and tissue-specific gene regulatory networks.BMC bioinformatics · 2018Article
- Epigenetic modifications in hyperhomocysteinemia: potential role in diabetic retinopathy and age-related macular degeneration.Oncotarget · 2018Article
- Hypermethylation: Causes and Consequences in Skeletal Muscle Myopathy.Journal of cellular biochemistry · 2017Review
- Prolonged cyclic strain inhibits human endothelial cell growth.Frontiers in bioscience (Elite edition) · 2016Article
- Homocysteine Induces Collagen I Expression by Downregulating Histone Methyltransferase G9a.PloS one · 2015Article
- Molecular mechanisms underlying the anti-proliferative and anti-migratory effects of folate on homocysteine-challenged rat aortic smooth muscle cells.British journal of pharmacology · 2013Article
- Homocysteine induces inflammatory transcriptional signaling in monocytes.Frontiers in bioscience (Landmark edition) · 2013Article
- Homocysteine impairs endothelial wound healing by activating metabotropic glutamate receptor 5.Microcirculation (New York, N.Y. : 1994) · 2012Article
- Homocysteine inhibits adipogenesis in 3T3-L1 preadipocytes.Experimental biology and medicine (Maywood, N.J.) · 2011Article
- Antecedent hydrogen sulfide elicits an anti-inflammatory phenotype in postischemic murine small intestine: role of heme oxygenase-1.American journal of physiology. Heart and circulatory physiology · 2011Article
- Review
- Regulation of homocysteine metabolism and methylation in human and mouse tissues.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Previously, it was reported that homocysteine (Hcy) specifically inhibits the growth of endothelial cells (ECs), suppresses Ras/mitogen-activated protein (MAP) signaling, and arrests cell growth at the G(1)/S transition of the cell cycle. The present study investigated the molecular mechanisms underlying this cell-cycle effect. Results showed that clinically relevant concentrations (50 microM) of Hcy significantly inhibited the expression of cyclin A messenger RNA (mRNA) in ECs in a dose- and time-dependent manner. G(1)/S-associated molecules that might account for this block were not changed, because Hcy did not affect mRNA and protein expression of cyclin D1 and cyclin E. Cyclin D1- and E-associated kinase activities were unchanged. In contrast, cyclin A-associated kinase activity and CDK2 kinase activity were markedly suppressed. Nuclear run-on assay demonstrated that Hcy decreased the transcription rate of the cyclin A gene but had no effect on the half-life of cyclin A mRNA. In transient transfection experiments, Hcy significantly inhibited cyclin A promoter activity in endothelial cells, but not in vascular smooth muscle cells. Finally, adenovirus-transduced cyclin A expression restored EC growth inhibition and overcame the S phase block imposed by Hcy. Taken together, these findings indicate that cyclin A is a critical functional target of Hcy-mediated EC growth inhibition.
Indexed as
Identifiers
11806997PMC5539868W1555819284What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.