ArticleBiophysical journal2002
Nonequilibrium phenomena in the phase separation of a two-component lipid bilayer.
Article in Biophysical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 80 citations in OpenAlex.
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- Crystallization around solid-like nanosized docks can explain the specificity, diversity, and stability of membrane microdomains.Frontiers in plant science · 2014Article
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- FRET in Membrane Biophysics: An Overview.Frontiers in physiology · 2011Article
- Effect of sphingomyelin headgroup size on molecular properties and interactions with cholesterol.Biophysical journal · 2010Article
- First-leaflet phase effect on properties of phospholipid bilayer formed through vesicle adsorption on LB monolayer.The Journal of membrane biology · 2010Article
- Dynamics of lipid domain formation: fluctuation analysis.Biochimica et biophysica acta · 2010Review
- GUV preparation and imaging: minimizing artifacts.Biochimica et biophysica acta · 2010Review
- Membrane microheterogeneity: Förster resonance energy transfer characterization of lateral membrane domains.European biophysics journal : EBJ · 2010Review
- Cholesterol-rich fluid membranes solubilize ceramide domains: implications for the structure and dynamics of mammalian intracellular and plasma membranes.The Journal of biological chemistry · 2009Article
- Enhancing protein stability by adsorption onto raftlike lipid domains.Journal of the American Chemical Society · 2009Article
- Lipid raft composition modulates sphingomyelinase activity and ceramide-induced membrane physical alterations.Biophysical journal · 2009Article
- Sphingomyelinase-induced domain shape relaxation driven by out-of-equilibrium changes of composition.Biophysical journal · 2009Article
- Formation of ceramide/sphingomyelin gel domains in the presence of an unsaturated phospholipid: a quantitative multiprobe approach.Biophysical journal · 2007Article
- Ceramide-domain formation and collapse in lipid rafts: membrane reorganization by an apoptotic lipid.Biophysical journal · 2007Article
- Coexisting stripe- and patch-shaped domains in giant unilamellar vesicles.Biochemistry · 2006Article
- Galactosylceramide domain microstructure: impact of cholesterol and nucleation/growth conditions.Biophysical journal · 2006Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lipid bilayers composed of two phospholipids with significant acyl-chain mismatch behave as nonideal mixtures. Although many of these systems are well characterized from the equilibrium point of view, studies concerning their nonequilibrium dynamics are still rare. The kinetics of lipid demixing (phase separation) was studied in model membranes (large unilamellar vesicles of 1:1 dilauroylphosphatidylcholine (C(12) acyl chain) and distearoylphosphatidylcholine (C(18) acyl chain)). For this purpose, photophysical techniques (fluorescence intensity, anisotropy, and fluorescence resonance energy transfer) were applied using suitable probes (gel phase probe trans-parinaric acid and fluid phase probe N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-dilauroylphosphatidylethanolamine). The nonequilibrium situation was induced by a sudden thermal quench from a one-fluid phase equilibrium situation (higher temperature) to the gel/fluid coexistence range (lower temperature). We verified that the attainment of equilibrium is a very slow process (occurs in a time scale of hours), leading to large domains at infinite time. The nonequilibrium structure stabilization is due essentially to temporarily rigidified C(12) chains in the interface between gel/fluid domains, which decrease the interfacial tension by acting as surfactants. The relaxation process becomes faster with the increase of the temperature drop. In addition, heterogeneity is already present in the supposed homogeneous fluid mixture at the higher temperature.
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