ArticleGenes & development2002
Isolating human transcription factor targets by coupling chromatin immunoprecipitation and CpG island microarray analysis.
Article in Genes & development, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 180 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
180 citing papers in PubMed.
- Examining NF-κB genomic interactions by ChIP-seq and CUT&Tag.bioRxiv : the preprint server for biology · 2024Article
- TRMT13 inhibits the growth of papillary thyroid cancer by targeting ANAPC4.Acta biochimica et biophysica Sinica · 2024Article
- Multi-Omics Profiling for Health.Molecular & cellular proteomics : MCP · 2023Review
- E2F regulation of theProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Aberrant Epigenetic Alteration of PAX1 Expression Contributes to Parathyroid Tumorigenesis.The Journal of clinical endocrinology and metabolism · 2022Observational
- ChIP-ping the branches of the tree: functional genomics and the evolution of eukaryotic gene regulation.Briefings in functional genomics · 2018Review
- Orphan CpG islands as alternative promoters.Transcription · 2018Article
- Detection of E2F-DNA Complexes Using Chromatin Immunoprecipitation Assays.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Analysis of the c-KIT Ligand Promoter Using Chromatin Immunoprecipitation.Journal of visualized experiments : JoVE · 2017Article
- Article
- Aberrant Retinoblastoma (RB)-E2F Transcriptional Regulation Defines Molecular Phenotypes of Osteosarcoma.The Journal of biological chemistry · 2015Article
- TODRA, a lncRNA at the RAD51 Locus, Is Oppositely Regulated to RAD51, and Enhances RAD51-Dependent DSB (Double Strand Break) Repair.PloS one · 2015Article
- Beyond the histone tale: HP1α deregulation in breast cancer epigenetics.Cancer biology & therapy · 2015Review
- Hematopoietic transcriptional mechanisms: from locus-specific to genome-wide vantage points.Experimental hematology · 2014Review
- ChIPseek, a web-based analysis tool for ChIP data.BMC genomics · 2014Article
- Fully automated high-throughput chromatin immunoprecipitation for ChIP-seq: identifying ChIP-quality p300 monoclonal antibodies.Scientific reports · 2014Article
- Large-scale quality analysis of published ChIP-seq data.G3 (Bethesda, Md.) · 2014Article
- Role of ChIP-seq in the discovery of transcription factor binding sites, differential gene regulation mechanism, epigenetic marks and beyond.Cell cycle (Georgetown, Tex.) · 2014Review
- The nuclear protein ALY binds to and modulates the activity of transcription factor E2F2.Molecular & cellular proteomics : MCP · 2013Article
- Article
120 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Previously, identification of promoters regulated by mammalian transcription factors has relied upon overexpression studies. Here we present the identification of a large set of promoters that are bound by E2F in physiological conditions. Probing a human CpG microarray with chromatin immunoprecipitated using an antibody to E2F4, we have identified 68 unique target loci; 15% are bidirectional promoters and 25% recruit E2F via a mechanism distinct from the defined consensus site. Interestingly, although E2F has been shown previously to regulate genes involved in cell cycle progression, many of the new E2F target genes encode proteins involved in DNA repair or recombination. We suggest that human CpG microarrays, in combination with chromatin immunoprecipitation, will allow rapid identification of target promoters for many mammalian transcription factors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.