ArticleInfection and immunity2002
Urease of enterohemorrhagic Escherichia coli: evidence for regulation by fur and a trans-acting factor.
Article in Infection and immunity, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 46 citations in OpenAlex.
- Fur-regulated urease contributes to the environmental adaptation ofMicrobiology spectrum · 2025Article
- Anti-persister efficacy of colistin and meropenem against uropathogenicMicrobiology (Reading, England) · 2023Article
- Breast milk urea as a nitrogen source for urease positive Bifidobacterium infantis.FEMS microbiology ecology · 2021Article
- The gluconeogenesis pathway is involved in maintenance of enterohaemorrhagic Escherichia coli O157:H7 in bovine intestinal content.PloS one · 2014Article
- Contribution of urease to colonization by Shiga toxin-producing Escherichia coli.Infection and immunity · 2012Article
- Modularity analysis based on predicted protein-protein interactions provides new insights into pathogenicity and cellular process of Escherichia coli O157:H7.Theoretical biology & medical modelling · 2011Article
- Functional and phylogenetic analysis of ureD in Shiga toxin-producing Escherichia coli.Journal of bacteriology · 2011Article
- The Citrobacter rodentium genome sequence reveals convergent evolution with human pathogenic Escherichia coli.Journal of bacteriology · 2010Article
- Edwardsiella ictaluri encodes an acid-activated urease that is required for intracellular replication in channel catfish (Ictalurus punctatus) macrophages.Applied and environmental microbiology · 2009Article
- Contributions of O island 48 to adherence of enterohemorrhagic Escherichia coli O157:H7 to epithelial cells in vitro and in ligated pig ileal loops.Applied and environmental microbiology · 2009Article
- Iron-responsive repression of urease expression in Helicobacter hepaticus is mediated by the transcriptional regulator Fur.Infection and immunity · 2007Article
- Microarray based comparison of two Escherichia coli O157:H7 lineages.BMC microbiology · 2006Article
- Distribution of the urease gene cluster among and urease activities of enterohemorrhagic Escherichia coli O157 isolates from humans.Journal of clinical microbiology · 2005Article
- Pathogenic Escherichia coli.Nature reviews. Microbiology · 2004Review
- stx1c Is the most common Shiga toxin 1 subtype among Shiga toxin-producing Escherichia coli isolates from sheep but not among isolates from cattle.Journal of clinical microbiology · 2003Article
- The gonococcal fur regulon: identification of additional genes involved in major catabolic, recombination, and secretory pathways.Journal of bacteriology · 2002Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
Recent genomic analyses of Escherichia coli O157:H7 strain EDL933 revealed two loci encoding urease gene homologues (ureDABCEFG), which are absent in nonpathogenic E. coli strain K-12. This report demonstrates that the cloned EDL933 ure gene cluster is capable of synthesizing urease in an E. coli DH5alpha background. However, when the gene fragment is transformed back into the native EDL933 background, the enzymatic activity of the cloned determinants is undetectable. We speculate that an unidentified trans-acting factor in enterohemorrhagic E. coli (EHEC) is responsible for this regulation of ure expression. In addition, Fur-like recognition sites are present in three independent O157:H7 isolates upstream of ureD and ureA. Enzymatic assays confirmed a difference in urease expression of cloned EHEC ure clusters in E. coli MC3100Deltafur. Likewise, interruption of fur in O157:H7 isolate IN1 significantly diminished urease activity. We propose that, similar to the function of Fur in regulating the acid response of Salmonella enterica serovar Typhimurium, it modulates urease expression in EHEC, perhaps contributing to the acid tolerance of the organism.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.