ArticleInfection and immunity2002
In vivo complementation of ureB restores the ability of Helicobacter pylori to colonize.
Article in Infection and immunity, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 58 citations in OpenAlex.
- Gastric Helicobacter species associated with dogs, cats and pigs: significance for public and animal health.Veterinary research · 2022Review
- Survival of Helicobacter pylori in gastric acidic territory.Helicobacter · 2017Review
- Characterization in Helicobacter pylori of a Nickel Transporter Essential for Colonization That Was Acquired during Evolution by Gastric Helicobacter Species.PLoS pathogens · 2016Article
- The Helicobacter pylori CZB Cytoplasmic Chemoreceptor TlpD Forms an Autonomous Polar Chemotaxis Signaling Complex That Mediates a Tactic Response to Oxidative Stress.Journal of bacteriology · 2016Article
- Helicobacter pylori CagA and IL-1β Promote the Epithelial-to-Mesenchymal Transition in a Nontransformed Epithelial Cell Model.Gastroenterology research and practice · 2016Article
- Evolution of Helicobacter: Acquisition by Gastric Species of Two Histidine-Rich Proteins Essential for Colonization.PLoS pathogens · 2015Article
- Helicobacter urease-induced activation of the TLR2/NLRP3/IL-18 axis protects against asthma.The Journal of clinical investigation · 2015Article
- Helicobacter pylori activates the TLR2/NLRP3/caspase-1/IL-18 axis to induce regulatory T-cells, establish persistent infection and promote tolerance to allergens.Gut microbes · 2015Review
- NMR study on small proteins from Helicobacter pylori for antibiotic target discovery: a review.Molecules (Basel, Switzerland) · 2013Review
- The role of ExbD in periplasmic pH homeostasis in Helicobacter pylori.Helicobacter · 2013Article
- Complex T cell interactions contribute to Helicobacter pylori gastritis in mice.Infection and immunity · 2013Article
- A supplemented soft agar chemotaxis assay demonstrates the Helicobacter pylori chemotactic response to zinc and nickel.Microbiology (Reading, England) · 2013Article
- Effects of human and porcine bile on the proteome of Helicobacter hepaticus.Proteome science · 2012Article
- Hierarchical regulation of the NikR-mediated nickel response in Helicobacter pylori.Nucleic acids research · 2011Article
- Surreptitious manipulation of the human host by Helicobacter pylori.Gut microbes · 2010Article
- The Helicobacter pylori GroES cochaperonin HspA functions as a specialized nickel chaperone and sequestration protein through its unique C-terminal extension.Journal of bacteriology · 2010Article
- Arginase II restricts host defense to Helicobacter pylori by attenuating inducible nitric oxide synthase translation in macrophages.Journal of immunology (Baltimore, Md. : 1950) · 2010Article
- Celecoxib inhibits Helicobacter pylori colonization-related factors.World journal of gastroenterology · 2010Article
- Iron-responsive repression of urease expression in Helicobacter hepaticus is mediated by the transcriptional regulator Fur.Infection and immunity · 2007Article
- NikR mediates nickel-responsive transcriptional repression of the Helicobacter pylori outer membrane proteins FecA3 (HP1400) and FrpB4 (HP1512).Infection and immunity · 2006Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
The objective of this study was to determine (i) if complementation of ureB-negative Helicobacter pylori restores colonization and (ii) if urease is a useful reporter for promoter activity in vivo. Strains used were M6, M6DeltaureB, and 10 recombinant derivatives of M6 or M6DeltaureB in which urease expression was under the control of different H. pylori promoters. Mice were orally inoculated with either the wild type or one of the mutant strains, and colonization, in vivo urease activity, and extent of gastritis were determined. Of eight M6DeltaureB recombinants tested, four colonized mice. Of those, three had the highest in vitro urease activity of any of the recombinants, significantly different from that of the noncolonizing mutants. The fourth colonizing recombinant, with ureB under control of the cag-15 promoter, had in vitro urease activity which did not differ significantly from the noncolonizing strains. In vivo, urease activities of the four colonizing transformants and the wild-type control were indistinguishable. There were no differences in gastritis or epithelial lesions between mice infected with M6 and those infected with the transformants. These results demonstrate that recovery of urease activity can restore colonizing ability to urease-negative H. pylori. They also suggest that cag-15 is upregulated in vivo, as was previously suggested by demonstrating that it is upregulated upon contact with epithelial cells. Finally, our results suggest that total urease activity and colonization density do not contribute to gastritis due to H. pylori.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.