ArticleInfection and immunity2002
Specific ligand binding attributable to individual epitopes of gonococcal transferrin binding protein A.
Article in Infection and immunity, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 32 citations in OpenAlex.
- Investigating the importance of selected surface-exposed loops in HpuB for hemoglobin binding and utilization byInfection and immunity · 2024Article
- Review
- A TonB dependent transporter YncD of Salmonella enterica Serovar Typhi possesses vaccine potential.World journal of microbiology & biotechnology · 2024Article
- Application of TonB-Dependent Transporters in Vaccine Development of Gram-Negative Bacteria.Frontiers in cellular and infection microbiology · 2020Review
- Beyond the Crystal Structure: Insight into the Function and Vaccine Potential of TbpA Expressed by Neisseria gonorrhoeae.Infection and immunity · 2015Article
- The transferrin-iron import system from pathogenic Neisseria species.Molecular microbiology · 2012Review
- The fbpABC operon is required for Ton-independent utilization of xenosiderophores by Neisseria gonorrhoeae strain FA19.Infection and immunity · 2011Article
- TonB-Dependent Transporters Expressed by Neisseria gonorrhoeae.Frontiers in microbiology · 2011Article
- Identification and characterization of gonococcal iron transport systems as potential vaccine antigens.Future microbiology · 2008Review
- Gonococcal transferrin binding protein chimeras induce bactericidal and growth inhibitory antibodies in mice.Vaccine · 2007Article
- High-affinity binding by the periplasmic iron-binding protein from Haemophilus influenzae is required for acquiring iron from transferrin.The Biochemical journal · 2007Article
- Identification of the iron-responsive genes of Neisseria gonorrhoeae by microarray analysis in defined medium.Journal of bacteriology · 2005Article
- Iron transport systems in Neisseria meningitidis.Microbiology and molecular biology reviews : MMBR · 2004Review
- Determination of surface-exposed, functional domains of gonococcal transferrin-binding protein A.Infection and immunity · 2004Article
- Immunogenicity of gonococcal transferrin binding proteins during natural infections.Infection and immunity · 2004Article
- Peptide-peptide interactions between human transferrin and transferrin-binding protein B from Moraxella catarrhalis.Journal of bacteriology · 2003Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The gonococcal transferrin receptor complex comprises two iron-regulated proteins, TbpA and TbpB. TbpA is essential for transferrin-iron uptake and is a TonB-dependent integral outer membrane protein. TbpB is thought to increase the efficiency of iron uptake from transferrin and is lipid modified and surface exposed. To evaluate the structure-function relationships in one of the components of the receptor, TbpA, we created constructs that fused individual putative loops of TbpA with amino-terminal affinity tags. The recombinant proteins were then overexpressed in Escherichia coli, and the fusions were recovered predominately from inclusion bodies. Inclusion body proteins were solubilized, and the epitope fusions were renatured by slow dialysis. To assess transferrin binding capabilities, the constructs were tested in a solid-phase dot blot assay followed by confirmatory quantitative chemiluminescent enzyme-linked immunosorbent assays. The constructs with only loop 5 and with loops 4 and 5 demonstrated dose-dependent specific ligand binding in spite of being out of the context of the intact receptor. The immunogenicities of individual TbpA-specific epitopes were investigated by generating rabbit polyclonal antisera against the fusion proteins. Most of the fusion proteins were immunogenic under these conditions, and the resulting sera recognized full-length TbpA in immunoblots. These results suggest that individual epitopes of TbpA are both immunogenic and functional with respect to ligand binding capabilities, and the vaccine implications of these findings are discussed.
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