ArticleInfection and immunity2002
The extracytoplasmic sigma factor, final sigma(E), is required for intracellular survival of nontypeable Haemophilus influenzae in J774 macrophages.
Article in Infection and immunity, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 53 citations in OpenAlex.
- Salmonella stress response systems as targets for anti-virulence strategies.BMC microbiology · 2025Article
- Maternal prenatal immunity, neonatal trained immunity, and early airway microbiota shape childhood asthma development.Allergy · 2022Article
- Nontypeable Haemophilus influenzae infection of pulmonary macrophages drives neutrophilic inflammation in severe asthma.Allergy · 2022Article
- The Alternative Sigma Factor RpoE2 Is Involved in the Stress Response to Hypochlorite andFrontiers in microbiology · 2021Article
- Interrupting the Conversation: Implications for Crosstalk Between Viral and Bacterial Infections in the Asthmatic Airway.Frontiers in allergy · 2021Review
- Dual RNASeq Reveals NTHi-Macrophage Transcriptomic Changes During Intracellular Persistence.Frontiers in cellular and infection microbiology · 2021Article
- Envelope stress responses: balancing damage repair and toxicity.Nature reviews. Microbiology · 2019Review
- Assay development and high-throughput screening for small molecule inhibitors of aDrug design, development and therapy · 2017Article
- Studies of the extracytoplasmic function sigma factor PG0162 in Porphyromonas gingivalis.Molecular oral microbiology · 2016Article
- Haemophilus influenzae responds to glucocorticoids used in asthma therapy by modulation of biofilm formation and antibiotic resistance.EMBO molecular medicine · 2015Article
- Differential transcriptional profile of Corynebacterium pseudotuberculosis in response to abiotic stresses.BMC genomics · 2014Article
- Identification of the Treponema pallidum subsp. pallidum TP0092 (RpoE) regulon and its implications for pathogen persistence in the host and syphilis pathogenesis.Journal of bacteriology · 2013Article
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- Identification of a novel anti-sigmaE factor in Neisseria meningitidis.BMC microbiology · 2010Article
- Cytotoxic T lymphocyte and natural killer cell responses to non-typeable Haemophilus influenzae.Clinical and experimental immunology · 2008Article
- Aminoglycosides affect intracellular Salmonella enterica serovars typhimurium and virchow.Antimicrobial agents and chemotherapy · 2008Article
- Effects of temperature on gene expression patterns in Leptospira interrogans serovar Lai as assessed by whole-genome microarrays.Infection and immunity · 2006Article
- The structural basis for promoter -35 element recognition by the group IV sigma factors.PLoS biology · 2006Article
- Role of sigma E-regulated genes in Escherichia coli uropathogenesis.Infection and immunity · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nontypeable Haemophilus influenzae (NTHi) causes a wide variety of respiratory tract infections in humans. It is capable of invading and surviving in epithelial cells and has also been shown to persist in macrophage-like cell line J774A.1. To determine the molecular mechanisms which enable NTHi to survive in an intracellular environment, differential display reverse transcriptase PCR was used to identify genes which were either induced or upregulated by NTHi residing in macrophages. Using this approach, we identified one transcript which was consistently amplified from intracellular NTHi cDNA. Nucleotide sequence analysis of this product revealed that it spanned the 3' and 5' ends of rpoE and rseB, respectively, which form part of the extracytoplasmic stress operon that encodes and regulates expression of alternate sigma factor sigma E (final sigma(E)). To confirm that expression of rpoE was upregulated following uptake of NTHi by macrophages, an rpoE-lacZ transcriptional fusion was constructed, and expression of beta-galactosidase activity in broth-grown NTHi was compared with expression of beta-galactosidase activity in intracellular NTHi. The level of beta-galactosidase activity in NTHi 4 h after phagocytosis by macrophages was found to be 100-fold higher than that of broth-grown organisms, suggesting that genes of the final sigma(E) regulon may be important for persistence of NTHi in mammalian cells. The hypothesis that final sigma(E) plays a role in the intracellular survival of NTHi was subsequently confirmed by the decreased ability of an rpoE insertion mutant to survive in macrophages.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.