ArticleAntimicrobial agents and chemotherapy2002
Type II topoisomerase quinolone resistance-determining regions of Aeromonas caviae, A. hydrophila, and A. sobria complexes and mutations associated with quinolone resistance.
Article in Antimicrobial agents and chemotherapy, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 72 citations in OpenAlex.
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- Genetic characterization and virulence determinants of multidrug-resistant NDM-1-producingFrontiers in microbiology · 2022Article
- Plasmid-Mediated Fluoroquinolone Resistance Genes in Quinolone-SusceptibleFrontiers in cellular and infection microbiology · 2022Article
- Molecular Typing, Antibiogram and PCR-RFLP Based Detection ofPathogens (Basel, Switzerland) · 2020Article
- Article
- Molecular analysis of type II topoisomerases of Aeromonas hydrophila isolated from fish and levofloxacin-induced resistant isolates in vitro.Folia microbiologica · 2016Article
- Detection of antibiotic resistance, virulence gene determinants and biofilm formation in Aeromonas species isolated from cattle.Environmental science and pollution research international · 2015Article
- Mechanisms of antimicrobial resistance in finfish aquaculture environments.Frontiers in microbiology · 2013Article
- Molecular characterization of tetracycline- and quinolone-resistant Aeromonas salmonicida isolated in Korea.Journal of veterinary science · 2011Article
- qnrVC-like gene located in a novel complex class 1 integron harboring the ISCR1 element in an Aeromonas punctata strain from an aquatic environment in Shandong Province, China.Antimicrobial agents and chemotherapy · 2010Article
- Article
- First gene cassettes of integrons as targets in finding adaptive genes in metagenomes.Applied and environmental microbiology · 2009Article
- Spontaneous quinolone resistance in the zoonotic serovar of Vibrio vulnificus.Applied and environmental microbiology · 2009Article
- Plasmid-mediated QnrS2 determinant from a clinical Aeromonas veronii isolate.Antimicrobial agents and chemotherapy · 2008Article
- Role of the AheABC efflux pump in Aeromonas hydrophila intrinsic multidrug resistance.Antimicrobial agents and chemotherapy · 2008Article
- Unexpected occurrence of plasmid-mediated quinolone resistance determinants in environmental Aeromonas spp.Emerging infectious diseases · 2008Article
- Analysis of the gyrA gene of clinical Yersinia ruckeri isolates with reduced susceptibility to quinolones.Applied and environmental microbiology · 2004Article
- Detection and identification of ciprofloxacin-resistant Yersinia pestis by denaturing high-performance liquid chromatography.Journal of clinical microbiology · 2003Article
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6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most Aeromonas strains isolated from two European rivers were previously found to be resistant to nalidixic acid. In order to elucidate the mechanism of this resistance, 20 strains of Aeromonas caviae (n = 10), A. hydrophila (n = 5), and A. sobria (n = 5) complexes, including 3 reference strains and 17 environmental isolates, were investigated. Fragments of the gyrA, gyrB, parC, and parE genes encompassing the quinolone resistance-determining regions (QRDRs) were amplified by PCR and sequenced. Results obtained for the six sensitive strains showed that the GyrA, GyrB, ParC, and ParE QRDR fragments of Aeromonas spp. were highly conserved (> or =96.1% identity), despite some genetic polymorphism; they were most closely related to those of Vibrio spp., Pseudomonas spp., and members of the family Enterobacteriaceae (72.4 to 97.1% homology). All 14 environmental resistant strains carried a point mutation in the GyrA QRDR at codon 83, leading to the substitution Ser-83-->Ile (10 strains) or Ser-83-->Arg. In addition, seven strains harbored a mutation in the ParC QRDR either at position 80 (five strains), generating a Ser-80-->Ile (three strains) or Ser-80-->Arg change, or at position 84, yielding a Glu-84-->Lys modification. No amino acid alterations were discovered in the GyrB and ParE QRDRs. Double gyrA-parC missense mutations were associated with higher levels of quinolone resistance compared with the levels associated with single gyrA mutations. The most resistant strains probably had an additional mechanism(s) of resistance, such as decreased accumulation of the drugs. Our data suggest that, in mesophilic Aeromonas spp., as in other gram-negative bacteria, gyrase and topoisomerase IV are the primary and secondary targets for quinolones, respectively.
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