ArticleProceedings of the National Academy of Sciences of the United States of America2002
Genomic structure and functional control of the Dlx3-7 bigene cluster.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 70 citations in OpenAlex.
- Review
- Review
- Functional Access to Neuron Subclasses in Rodent and Primate Forebrain.Cell reports · 2019Article
- Conserved noncoding sequences conserve biological networks and influence genome evolution.Heredity · 2018Article
- Homeobox transcription factor DLX4 is not necessary for skin development and homeostasis.Experimental dermatology · 2018Article
- A discrete transition zone organizes the topological and regulatory autonomy of the adjacent tfap2c and bmp7 genes.PLoS genetics · 2015Article
- Sequence analyses of the distal-less homeobox gene family in East African cichlid fishes reveal signatures of positive selection.BMC evolutionary biology · 2013Article
- In vivo impact of Dlx3 conditional inactivation in neural crest-derived craniofacial bones.Journal of cellular physiology · 2013Article
- Non-parsimonious evolution of hagfish Dlx genes.BMC evolutionary biology · 2013Article
- Article
- Theria-specific homeodomain and cis-regulatory element evolution of the Dlx3-4 bigene cluster in 12 different mammalian species.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2012Article
- Annelid Distal-less/Dlx duplications reveal varied post-duplication fates.BMC evolutionary biology · 2011Article
- Expression of the Distalless-B gene in Ciona is regulated by a pan-ectodermal enhancer module.Developmental biology · 2011Article
- Large-scale analysis of the regulatory architecture of the mouse genome with a transposon-associated sensor.Nature genetics · 2011Article
- Article
- Article
- Enhancers of GnRH transcription embedded in an upstream gene use homeodomain proteins to specify hypothalamic expression.Molecular endocrinology (Baltimore, Md.) · 2010Article
- The relationship between dlx and gad1 expression indicates highly conserved genetic pathways in the zebrafish forebrain.Developmental dynamics : an official publication of the American Association of Anatomists · 2010Article
- Dlx3 is a crucial regulator of hair follicle differentiation and cycling.Development (Cambridge, England) · 2008Article
- Molecular consequences of a frameshifted DLX3 mutant leading to Tricho-Dento-Osseous syndrome.The Journal of biological chemistry · 2008Article
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
Abstract
The Dlx genes are involved in early vertebrate morphogenesis, notably of the head. The six Dlx genes of mammals are arranged in three convergently transcribed bigene clusters. In this study, we examine the regulation of the Dlx3-7 cluster of the mouse. We obtained and sequenced human and mouse P1 clones covering the entire Dlx3-7 cluster. Comparative analysis of the human and mouse sequences revealed several highly conserved noncoding regions within 30 kb of the Dlx3-7-coding regions. These conserved elements were located both 5' of the coding exons of each gene and in the intergenic region 3' of the exons, suggesting that some enhancers might be shared between genes. We also found that the protein sequence of Dlx7 is evolving more rapidly than that of Dlx3. We conducted a functional study of the 79-kb mouse genomic clone to locate cis-element activity able to reproduce the endogenous expression pattern by using transgenic mice. We inserted a lacZ reporter gene into the first exon of the Dlx3 gene by using homologous recombination in yeast. Strong lacZ expression in embryonic (E) stage E9.5 and E10.5 mouse embryos was found in the limb buds and first and second visceral arches, consistent with the endogenous Dlx3 expression pattern. This result shows that the 79-kb region contains the major cis-elements required to direct the endogenous expression of Dlx3 at stage E10.5. To test for enhancer location, we divided the construct in the mid-intergenic region and injected the Dlx3 gene portion. This shortened fragment lacking Dlx7-flanking sequences is able to drive expression in the limb buds but not in the visceral arches. This observation is consistent with a cis-regulatory enhancer-sharing model within the Dlx bigene cluster.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.