ArticleThe Journal of physiology2002
Prolonged increase in ciliary beat frequency after short-term purinergic stimulation in human airway epithelial cells.
Article in The Journal of physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 83 citations in OpenAlex.
- Pharmacological regulation of airway ciliary motility: endogenous mediators, pharmaceutical agents, and natural compounds with a focus on ginsenoside Rd.Journal of natural medicines · 2026Review
- Structure and function relationships of mucociliary clearance in human and rat airways.Nature communications · 2025Article
- Structure and Function Relationships of Mucociliary Clearance in Human and Rat Airways.bioRxiv : the preprint server for biology · 2025Article
- PMFrontiers in public health · 2025Article
- Ambroxol-Enhanced Frequency and Amplitude of Beating Cilia Controlled by a Voltage-Gated CaInternational journal of molecular sciences · 2023Article
- A fixed 20:1 combination of cafedrine/theodrenaline increases cytosolic CaScientific reports · 2023Article
- Echinocandins Accelerate Particle Transport Velocity in the Murine Tracheal Epithelium: Dependency on Intracellular CaAntimicrobial agents and chemotherapy · 2021Article
- Drug-induced ciliogenesis in pancreatic cancer cells is facilitated by the secreted ATP-purinergic receptor signaling pathway.Oncotarget · 2018Article
- CALHM1-Mediated ATP Release and Ciliary Beat Frequency Modulation in Nasal Epithelial Cells.Scientific reports · 2017Article
- Possible contribution of pannexin-1 to capsaicin-induced ATP release in rat nasal columnar epithelial cells.Channels (Austin, Tex.) · 2017Article
- Connexins, Pannexins, and Their Channels in Fibroproliferative Diseases.The Journal of membrane biology · 2016Review
- Soluble adenylyl cyclase mediates hydrogen peroxide-induced changes in epithelial barrier function.Respiratory research · 2016Article
- How does rhinovirus cause the common cold cough?BMJ open respiratory research · 2016Article
- The Endoplasmic Reticulum Resident Protein AGR3. Required for Regulation of Ciliary Beat Frequency in the Airway.American journal of respiratory cell and molecular biology · 2015Article
- Airway hydration and COPD.Cellular and molecular life sciences : CMLS · 2015Review
- Hydrogen peroxide stimulation of CFTR reveals an Epac-mediated, soluble AC-dependent cAMP amplification pathway common to GPCR signalling.British journal of pharmacology · 2015Article
- Soluble adenylyl cyclase in health and disease.Biochimica et biophysica acta · 2014Review
- A soluble adenylyl cyclase form targets to axonemes and rescues beat regulation in soluble adenylyl cyclase knockout mice.American journal of respiratory cell and molecular biology · 2014Article
- Epithelial cell culture from human adenoids: a functional study model for ciliated and secretory cells.BioMed research international · 2013Article
- Identification of pannexins in rat nasal mucosa.Allergy & rhinology (Providence, R.I.) · 2013Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Stimulation of ovine airway epithelial cells with 10 microM ATP for 1 min at 25 degrees C transiently increased both cytoplasmic calcium (fura-2 epifluorescence microscopy) and ciliary beat frequency (CBF; differential interference contrast microscopy) with a similar time course. Identical purinergic stimulation of human airway epithelial cells at 25 or 35 degrees C, however, lead to an increase in CBF that outlasted the calcium transient at least 20 min. While a nitric oxide synthase inhibitor had no effect, pre-treatment of human cells with inhibitors of cAMP-dependent kinase (PKA), 10 microM myristoylated PKA-inhibitory peptide and 1 microM KT-5720, as well as an inhibitor of adenylyl cyclase, 1 mM SQ22536, blocked the prolonged, but not calcium-coupled CBF increase. Addition of PKA inhibitors after purinergic stimulation only partially reduced CBF from its elevated plateau. Prolonged CBF increases did not depend on adenosine production as 10 microM UTP had an effect similar to ATP and 8-sulphophenyl-theophylline did not block them. After increasing human CBF in a PKA-dependent manner to a stable plateau with forskolin (10 microM), ATP caused only a transient, calcium-coupled CBF increase. Calcium transients were necessary for both short-term and prolonged CBF changes as ATP failed to produce CBF increases after emptying calcium stores with 1 microM thapsigargin. These data suggest that in human, but not ovine airway epithelial cells, ATP-induced calcium transients activate a signalling cascade including adenylyl cyclase and PKA. The resulting prolonged CBF stimulation does not rely only on PKA activity, suggesting that the decay of CBF is influenced by ciliary phosphatase activity.
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