ArticleThe Journal of physiology2002
Tubular system volume changes in twitch fibres from toad and rat skeletal muscle assessed by confocal microscopy.
Article in The Journal of physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 32 citations in OpenAlex.
- Measurements of basal d-glucose transport through GLUT1 across the intact plasma membrane of isolated segments from single fast- and slow-twitch skeletal muscle fibres of rat.Acta physiologica (Oxford, England) · 2022Article
- The ins and outs of acid-base transport in skeletal muscle.The Journal of general physiology · 2018Article
- NHE- and diffusion-dependent proton fluxes across the tubular system membranes of fast-twitch muscle fibers of the rat.The Journal of general physiology · 2018Article
- Human skeletal muscle plasmalemma alters its structure to change its CaNature communications · 2017Article
- A quantitative description of tubular system Ca(2+) handling in fast- and slow-twitch muscle fibres.The Journal of physiology · 2016Article
- Excitation of skeletal muscle is a self-limiting process, due to run-down of Na+, K+ gradients, recoverable by stimulation of the Na+, K+ pumps.Physiological reports · 2015Article
- The determinants of transverse tubular volume in resting skeletal muscle.The Journal of physiology · 2014Article
- Longitudinal and transversal propagation of excitation along the tubular system of rat fast-twitch muscle fibres studied by high speed confocal microscopy.The Journal of physiology · 2012Article
- Strain transfer in ventricular cardiomyocytes to their transverse tubular system revealed by scanning confocal microscopy.Biophysical journal · 2011Article
- The accessibility and interconnectivity of the tubular system network in toad skeletal muscle.The Journal of physiology · 2008Article
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- Dynamic regulation of the large exocytotic fusion pore in pancreatic acinar cells.Molecular biology of the cell · 2007Article
- Store-operated Ca2+ entry during intracellular Ca2+ release in mammalian skeletal muscle.The Journal of physiology · 2007Article
- Conduction velocities in amphibian skeletal muscle fibres exposed to hyperosmotic extracellular solutions.Journal of muscle research and cell motility · 2007Article
- Alterations in triad ultrastructure following repetitive stimulation and intracellular changes associated with exercise in amphibian skeletal muscle.Journal of muscle research and cell motility · 2007Article
- Effect of repetitive stimulation on cell volume and its relationship to membrane potential in amphibian skeletal muscle.Pflugers Archiv : European journal of physiology · 2006Article
- Tubular system excitability: an essential component of excitation-contraction coupling in fast-twitch fibres of vertebrate skeletal muscle.Journal of muscle research and cell motility · 2006Review
- Regulation of Ca2+ sparks by Ca2+ and Mg2+ in mammalian and amphibian muscle. An RyR isoform-specific role in excitation-contraction coupling?The Journal of general physiology · 2004Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The volume of the extracellular compartment (tubular system) within intact muscle fibres from cane toad and rat was measured under various conditions using confocal microscopy. Under physiological conditions at rest, the fractional volume of the tubular system (t-sys(Vol)) was 1.38 +/- 0.09 % (n = 17), 1.41 +/- 0.09 % (n = 12) and 0.83 +/- 0.07 % (n = 12) of the total fibre volume in the twitch fibres from toad iliofibularis muscle, rat extensor digitorum longus muscle and rat soleus muscle, respectively. In toad muscle fibres, the t-sys(Vol) decreased by 30 % when the tubular system was fully depolarized and decreased by 15 % when membrane cholesterol was depleted from the tubular system with methyl-beta-cyclodextrin but did not change as the sarcomere length was changed from 1.93 to 3.30 microm. There was also an increase by 30 % and a decrease by 25 % in t-sys(Vol) when toad fibres were equilibrated in solutions that were 2.5-fold hypertonic and 50 % hypotonic, respectively. When the changes in total fibre volume were taken into consideration, the t-sys(Vol) expressed as a percentage of the isotonic fibre volume did actually decrease as tonicity increased, revealing that the tubular system in intact fibres cannot be compressed below 0.9 % of the isotonic fibre volume. The results can be explained in terms of forces acting at the level of the tubular wall. These observations have important physiological implications showing that the tubular system is a dynamic membrane structure capable of changing its volume in response to the membrane potential, cholesterol depletion and osmotic stress but not when the sarcomere length is changed in resting muscle.
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