ArticleThe Journal of physiology2002
The role of the L-type Ca(2+) channel in refilling functional intracellular Ca(2+) stores in guinea-pig detrusor smooth muscle.
Article in The Journal of physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- In Silico Electrophysiological Investigation of Transient Receptor Potential Melastatin-4 Ion Channel Biophysics to Study Detrusor Overactivity.International journal of molecular sciences · 2024Article
- Adreno-Muscarinic Synergy of Contractile Responses From Human Hyperplastic Prostate.International neurourology journal · 2024Article
- Preparation and Utilization of Freshly Isolated Human Detrusor Smooth Muscle Cells for Characterization of 9-Phenanthrol-Sensitive Cation Currents.Journal of visualized experiments : JoVE · 2020Article
- Constitutively active HCN channels constrain detrusor excitability and modulate evoked contractions of human bladder.American journal of clinical and experimental urology · 2020Article
- Elementary calcium signaling in arterial smooth muscle.Channels (Austin, Tex.) · 2019Review
- Ultrasound induces contraction of the bladder smooth muscle.International urology and nephrology · 2016Article
- A 'calcium capacitor' shapes cholinergic inhibition of cochlear hair cells.The Journal of physiology · 2014Review
- T-type Ca2+ channels and the urinary and male genital tracts.Pflugers Archiv : European journal of physiology · 2014Review
- Expression and function of K(V)2-containing channels in human urinary bladder smooth muscle.American journal of physiology. Cell physiology · 2012Article
- Aging differentially modifies agonist-evoked mouse detrusor contraction and calcium signals.Age (Dordrecht, Netherlands) · 2011Article
- In airways ATP refills sarcoplasmic reticulum via P2X smooth muscle receptors and induces contraction through P2Y epithelial receptors.Pflugers Archiv : European journal of physiology · 2011Article
- Nerve-released acetylcholine contracts urinary bladder smooth muscle by inducing action potentials independently of IP3-mediated calcium release.American journal of physiology. Regulatory, integrative and comparative physiology · 2010Article
- Cholinergic-induced Ca2+ signaling in interstitial cells of Cajal from the guinea pig bladder.American journal of physiology. Renal physiology · 2008Article
- Molecular mechanisms of detrusor and corporal myocyte contraction: identifying targets for pharmacotherapy of bladder and erectile dysfunction.British journal of pharmacology · 2006Review
- Purinergic regulation of guinea pig suburothelial myofibroblasts.The Journal of physiology · 2004Article
- SERCA pump optimizes Ca2+ release by a mechanism independent of store filling in smooth muscle cells.Biophysical journal · 2003Article
- Mechanisms of excitatory neuromuscular transmission in the guinea-pig urinary bladder.The Journal of physiology · 2000Article
- Electrophysiological properties of the bladder.International urogynecology journal and pelvic floor dysfunction · 1998Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The transient rise of intracellular Ca(2+) in detrusor smooth muscle cells is due to the release of Ca(2+) from intracellular stores. However, it is not known how store refilling is maintained at a constant level to ensure constancy of the contractile response. The aim of these experiments was to characterise the role of L-type Ca(2+) channels in refilling. Experiments used isolated guinea-pig detrusor myocytes and store Ca(2+) content was estimated by measuring the magnitude of change to the intracellular [Ca(2+)] ([Ca(2+)](i)) after application of caffeine or carbachol using epifluorescence microscopy. Membrane potential was controlled when necessary by voltage clamp. After Ca(2+) stores were emptied they refilled with an exponential time course, with a time constant of 88 s. The value of the time constant was similar to that of the undershoot of [Ca(2+)](i) following store Ca(2+) release. The degree of store filling was enhanced by maintained depolarisation, or by transient depolarising pulses, and attenuated by L-type Ca(2+) channel antagonists. Inhibition of the sarcoplasmic reticular Ca(2+)-ATPase prevented refilling. Reduction of the resting [Ca(2+)](i) was accompanied by membrane depolarisation; under voltage clamp reduction of [Ca(2+)](i) decreased the number and magnitude of spontaneous transient outward currents. Ca(2+) release from intracellular stores, elicited by caffeine or carbachol, is independent of membrane potential under physiological conditions. However, store refilling occurs via Ca(2+) influx through L-type Ca(2+) channels. Ca(2+) influx is regulated by a feedback mechanism whereby a fall of [Ca(2+)](i) reduces the activity of Ca(2+)-activated K(+) channels, causing cell depolarisation and an enhancement of L-type Ca(2+) channel conductance.
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