ArticleJournal of bacteriology2002
Molecular characterization and transcriptional analysis of adhE2, the gene encoding the NADH-dependent aldehyde/alcohol dehydrogenase responsible for butanol production in alcohologenic cultures of Clostridium acetobutylicum ATCC 824.
Article in Journal of bacteriology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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48 citing papers in PubMed, 161 citations in OpenAlex.
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- Engineering Cupriavidus necator H16 for the autotrophic production of (R)-1,3-butanediol.Metabolic engineering · 2021Article
- Production of the biocommodities butanol and acetone from methanol with fluorescent FAST-tagged proteins using metabolically engineered strains of Eubacterium limosum.Biotechnology for biofuels · 2021Article
- Engineering nature for gaseous hydrocarbon production.Microbial cell factories · 2020Review
- CRISPR/Cas9-mediated engineering of Escherichia coli for n-butanol production from xylose in defined medium.Journal of industrial microbiology & biotechnology · 2019Article
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- Discovery and implementation of a novel pathway forBiotechnology for biofuels · 2019Article
- Functional Expression of the Clostridium ljungdahlii Acetyl-Coenzyme A Synthase in Clostridium acetobutylicum as Demonstrated by a NovelApplied and environmental microbiology · 2018Article
- Consolidated bioprocessing of butanol production from xylan by a thermophilic and butanologenicBiotechnology for biofuels · 2018Article
- Metabolic engineering ofBiotechnology for biofuels · 2017Article
- Genome-directed analysis of prophage excision, host defence systems, and central fermentative metabolism in Clostridium pasteurianum.Scientific reports · 2016Article
- Elucidating the contributions of multiple aldehyde/alcohol dehydrogenases to butanol and ethanol production in Clostridium acetobutylicum.Scientific reports · 2016Article
- Self-regulated 1-butanol production inBiotechnology for biofuels · 2016Article
- Elucidation of the roles of adhE1 and adhE2 in the primary metabolism of Clostridium acetobutylicum by combining in-frame gene deletion and a quantitative system-scale approach.Biotechnology for biofuels · 2016Article
- Development of a plasmid addicted system that is independent of co-inducers, antibiotics and specific carbon source additions for bioproduct (1-butanol) synthesis inMetabolic engineering communications · 2015Article
- A Quantitative System-Scale Characterization of the Metabolism of Clostridium acetobutylicum.mBio · 2015Article
- Transcriptional analysis of micronutrient zinc-associated response for enhanced carbohydrate utilization and earlier solventogenesis in Clostridium acetobutylicum.Scientific reports · 2015Article
- Alcohol Selectivity in a Synthetic Thermophilic n-Butanol Pathway Is Driven by Biocatalytic and Thermostability Characteristics of Constituent Enzymes.Applied and environmental microbiology · 2015Article
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Authors and funding
6 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The adhE2 gene of Clostridium acetobutylicum ATCC 824, coding for an aldehyde/alcohol dehydrogenase (AADH), was characterized from molecular and biochemical points of view. The 2,577-bp adhE2 codes for a 94.4-kDa protein. adhE2 is expressed, as a monocistronic operon, in alcohologenic cultures and not in solventogenic cultures. Primer extension analysis identified two transcriptional start sites 160 and 215 bp upstream of the adhE2 start codon. The expression of adhE2 from a plasmid in the DG1 mutant of C. acetobutylicum, a mutant cured of the pSOL1 megaplasmid, restored butanol production and provided elevated activities of NADH-dependent butyraldehyde and butanol dehydrogenases. The recombinant AdhE2 protein expressed in E. coli as a Strep-tag fusion protein and purified to homogeneity also demonstrated NADH-dependent butyraldehyde and butanol dehydrogenase activities. This is the second AADH identified in C. acetobutylicum ATCC 824, and to our knowledge this is the first example of a bacterium with two AADHs. It is noteworthy that the two corresponding genes, adhE and adhE2, are carried by the pSOL1 megaplasmid of C. acetobutylicum ATCC 824.
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