ArticleNucleic acids research2002
DNA microarrays with PAMAM dendritic linker systems.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 182 citations in OpenAlex.
- Visual detection of bacterial DNA using activated paper stripe.Mikrochimica acta · 2019Article
- Immobilization techniques for microarray: challenges and applications.Sensors (Basel, Switzerland) · 2014Review
- Isolation and amplification of mRNA within a simple microfluidic lab on a chip.Analytical chemistry · 2014Article
- Dendrimers: synthesis, applications, and properties.Nanoscale research letters · 2014Article
- Drop drying on surfaces determines chemical reactivity - the specific case of immobilization of oligonucleotides on microarrays.BMC biophysics · 2013Article
- A dendrimer-based immunosensor for improved capture and detection of tumor necrosis factor-α cytokine.Analytica chimica acta · 2012Article
- High-Q optical sensors for chemical and biological analysis.Analytical chemistry · 2012Review
- High sensitivity carbon nanotube based electrochemiluminescence sensor array.Biosensors & bioelectronics · 2012Article
- Chemistries for patterning robust DNA microbarcodes enable multiplex assays of cytoplasm proteins from single cancer cells.Chemphyschem : a European journal of chemical physics and physical chemistry · 2010Article
- Direct and rapid detection of RNAs on a novel RNA microchip.Chembiochem : a European journal of chemical biology · 2010Article
- Multifunctional Dendrimer-templated Antibody Presentation on Biosensor Surfaces for Improved Biomarker Detection.Advanced functional materials · 2010Article
- Bioconjugation techniques for microfluidic biosensors.Analytical and bioanalytical chemistry · 2009Article
- Stripping custom microRNA microarrays and the lessons learned about probe-slide interactions.Analytical biochemistry · 2009Article
- Multiple primer extension by DNA polymerase on a novel plastic DNA array coated with a biocompatible polymer.Nucleic acids research · 2007Article
- Increasing the sensitivity of DNA microarrays by metal-enhanced fluorescence using surface-bound silver nanoparticles.Nucleic acids research · 2007Article
- Position of the fluorescent label is a crucial factor determining signal intensity in microarray hybridizations.Nucleic acids research · 2005Article
- Construction of oligonucleotide arrays on a glass surface using a heterobifunctional reagent, N-(2-trifluoroethanesulfonatoethyl)-N-(methyl)-triethoxysilylpropyl-3-amine (NTMTA).Nucleic acids research · 2004Article
- Impact of surface chemistry and blocking strategies on DNA microarrays.Nucleic acids research · 2003Article
- Dendrimeric coating of glass slides for sensitive DNA microarrays analysis.Nucleic acids research · 2003Article
- Optimization strategies for DNA microarray-based detection of bacteria with 16S rRNA-targeting oligonucleotide probes.Applied and environmental microbiology · 2003Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The DNA microarray-based analysis of single nucleotide polymorphisms (SNPs) is important for the correlation of genetic variations and individual phenotypes, and for locating disease-causing genes. To facilitate the development of surfaces suitable for immobilization of oligonucleotides, we report here a novel method for the surface immobilization of DNA using pre-fabricated polyamidoamine (PAMAM) starburst dendrimers as mediator moieties. Dendrimers containing 64 primary amino groups in their outer sphere are covalently attached to silylated glass supports and, subsequently, the dendritic macromolecules are modified with glutaric anhydride and activated with N-hydroxysuccinimide. As a result of the dendritic PAMAM linker system the surfaces reveal both a very high immobilization efficiency for amino-modified DNA-oligomers, and also a remarkable high stability during repeated regeneration and re-using cycles. The performance of dendrimer-based DNA microarrays in the discrimination of SNPs is demonstrated.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.