ArticleNucleic acids research2002
A new class of homogeneous nucleic acid probes based on specific displacement hybridization.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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Who cites it
64 citing papers in PubMed, 278 citations in OpenAlex.
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- Expanding detection windows for discriminating single nucleotide variants using rationally designed DNA equalizer probes.Nature communications · 2020Article
- DNA nanotechnology assisted nanopore-based analysis.Nucleic acids research · 2020Review
- Context-aware seeds for read mapping.Algorithms for molecular biology : AMB · 2020Article
- Optimizing peptide nucleic acid probes for hybridization-based detection and identification of bacterial pathogens.The Analyst · 2019Article
- Fluorescent DNA Biosensor for Single-Base Mismatch Detection Assisted by Cationic Comb-Type Copolymer.Molecules (Basel, Switzerland) · 2019Article
- Nucleic Acid Strand Displacement with Synthetic mRNA Inputs in Living Mammalian Cells.ACS synthetic biology · 2018Article
- Voltammetric determination of the Alzheimer's disease-related ApoE 4 gene from unamplified genomic DNA extracts by ferrocene-capped gold nanoparticles.Mikrochimica acta · 2018Article
- Simulation-guided engineering of an enzyme-powered three dimensional DNA nanomachine for discriminating single nucleotide variants.Chemical science · 2018Article
- Individually addressable and dynamic DNA gates for multiplexed cell sorting.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- A red-shifted two-photon-only caging group for three-dimensional photorelease.Chemical science · 2018Article
- Detection of cofilin mRNA by hybridization-sensitive double-stranded fluorescent probes.RSC advances · 2018Article
- Displacement and hybridization reactions in aptamer-functionalized hydrogels for biomimetic protein release and signal transduction.Chemical science · 2017Article
- Ultra-specific discrimination of single-nucleotide mutations using sequestration-assisted molecular beacons.Chemical science · 2017Article
4 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a new class of probes for homogeneous nucleic acid detection based on the proposed displacement hybridization. Our probes consist of two complementary oligodeoxyribonucleotides of different length labeled with a fluorophore and a quencher in close proximity in the duplex. The probes on their own are quenched, but they become fluorescent upon displacement hybridization with the target. These probes display complete discrimination between a perfectly matched target and single nucleotide mismatch targets. A comparison of double-stranded probes with corresponding linear probes confirms that the presence of the complementary strand significantly enhances their specificity. Using four such probes labeled with different color fluorophores, each designed to recognize a different target, we have demonstrated that multiple targets can be distinguished in the same solution, even if they differ from one another by as little as a single nucleotide. Double-stranded probes were used in real-time nucleic acid amplifications as either probes or as primers. In addition to its extreme specificity and flexibility, the new class of probes is simple to design and synthesize, has low cost and high sensitivity and is accessible to a wide range of labels. This class of probes should find applications in a variety of areas wherever high specificity of nucleic acid hybridization is relevant.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.