ArticleNucleic acids research2002
Rolling-circle amplification under topological constraints.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Past, present, and future strategies for detecting and quantifying circular RNA variants.The FEBS journal · 2025Review
- In situ imaging of intracellular miRNAs in tumour cells by branched hybridisation chain reaction.Cell proliferation · 2024Article
- Lateral flow assays: Progress and evolution of recent trends in point-of-care applications.Materials today. Bio · 2024Review
- Oxime formation coordination-directed detection of genome-wide thymine oxides with nanogram-scale sample input.Chemical science · 2022Article
- Single-stranded DNA aptamer-based rolling circle amplification as anti-chickenVeterinary world · 2022Article
- Rolling circle RNA synthesis catalyzed by RNA.eLife · 2022Article
- Recent advances in sensitivity enhancement for lateral flow assay.Mikrochimica acta · 2021Review
- Chemical approaches to discover the full potential of peptide nucleic acids in biomedical applications.Beilstein journal of organic chemistry · 2021Review
- Detection of Prorocentrum minimum by hyperbranched rolling circle amplification coupled with lateral flow dipstick.Environmental science and pollution research international · 2020Article
- Current and Future Perspectives on Isothermal Nucleic Acid Amplification Technologies for Diagnosing Infections.Infection and drug resistance · 2020Review
- Characterization of Binding of Magnetic Nanoparticles to Rolling Circle Amplification Products by Turn-On Magnetic Assay.Biosensors · 2019Article
- Terminal hairpin in oligonucleotide dominantly prioritizes intramolecular cyclization by T4 ligase over intermolecular polymerization: an exclusive methodology for producing ssDNA rings.Nucleic acids research · 2018Article
- Cyclization of secondarily structured oligonucleotides to single-stranded rings by usingRSC advances · 2018Article
- Rolling circle amplification shows a sinusoidal template length-dependent amplification bias.Nucleic acids research · 2018Article
- A paper and plastic device for the combined isothermal amplification and lateral flow detection of Plasmodium DNA.Malaria journal · 2015Article
- Sensitive isothermal detection of nucleic-acid sequence by primer generation-rolling circle amplification.Nucleic acids research · 2009Article
- Labeling of unique sequences in double-stranded DNA at sites of vicinal nicks generated by nicking endonucleases.Nucleic acids research · 2008Article
- Detection of low-copy-number genomic DNA sequences in individual bacterial cells by using peptide nucleic acid-assisted rolling-circle amplification and fluorescence in situ hybridization.Applied and environmental microbiology · 2007Article
- Monitoring single-stranded DNA secondary structure formation by determining the topological state of DNA catenanes.Biophysical journal · 2006Article
- Base-stacking and base-pairing contributions into thermal stability of the DNA double helix.Nucleic acids research · 2006Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
We have performed rolling-circle amplification (RCA) reactions on three DNA templates that differ distinctly in their topology: an unlinked DNA circle, a linked DNA circle within a pseudorotaxane-type structure and a linked DNA circle within a catenane. In the linked templates, the single-stranded circle (dubbed earring probe) is threaded, with the aid of two peptide nucleic acid openers, between the two strands of double-stranded DNA (dsDNA). We have found that the RCA efficiency of amplification was essentially unaffected when the linked templates were employed. By showing that the DNA catenane remains intact after RCA reactions, we prove that certain DNA polymerases can carry out the replicative synthesis under topological constraints allowing detection of several hundred copies of a dsDNA marker without DNA denaturation. Our finding may have practical implications in the area of DNA diagnostics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.