ArticleGut2002
Intimin type influences the site of human intestinal mucosal colonisation by enterohaemorrhagic Escherichia coli O157:H7.
Article in Gut, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 119 citations in OpenAlex.
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- Colonizing foreign terrain: Insights into bacterial enteropathogens.Biomedical journal · 2023Article
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- A pathogen-specific sRNA influences enterohemorrhagic Escherichia coli fitness and virulence in part by direct interaction with the transcript encoding the ethanolamine utilization regulatory factor EutR.Nucleic acids research · 2021Article
- An Overview of the Elusive Passenger in the Gastrointestinal Tract of Cattle: The Shiga Toxin ProducingMicroorganisms · 2020Review
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- The StcE metalloprotease of enterohaemorrhagic Escherichia coli reduces the inner mucus layer and promotes adherence to human colonic epithelium ex vivo.Cellular microbiology · 2017Article
- Conserved Features in the Structure, Mechanism, and Biogenesis of the Inverse Autotransporter Protein Family.Genome biology and evolution · 2016Article
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- Type Three Secretion System in Attaching and Effacing Pathogens.Frontiers in cellular and infection microbiology · 2016Review
- Enterohemorrhagic Escherichia coli senses low biotin status in the large intestine for colonization and infection.Nature communications · 2015Article
- In vitro adhesion properties of Shiga toxin-producing Escherichia coli isolated from cattle, food, and humans.Frontiers in microbiology · 2015Article
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- Potentially pathogenic Escherichia coli can form a biofilm under conditions relevant to the food production chain.Applied and environmental microbiology · 2014Article
- Exploiting the power of OMICS approaches to produce E. coli O157 vaccines.Gut microbes · 2014Article
- Pathogenesis of human enterovirulent bacteria: lessons from cultured, fully differentiated human colon cancer cell lines.Microbiology and molecular biology reviews : MMBR · 2013Review
- Adhesion of Diarrheagenic Escherichia coli and Inhibition by Glycocompounds Engaged in the Mucosal Innate Immunity.Biology · 2013Article
- Proteomic View of Interactions of Shiga Toxin-Producing Escherichia coli with the Intestinal Environment in Gnotobiotic Piglets.PloS one · 2013Article
- Enteropathogenic and enterohemorrhagic E. coli: ecology, pathogenesis, and evolution.Frontiers in cellular and infection microbiology · 2013Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundEnterohaemorrhagic (EHEC) and enteropathogenic (EPEC) Escherichia coli epithelial cell adhesion is characterised by intimate attachment, and attaching and effacing (A/E) lesion formation. This event is mediated in part by intimin binding to another bacterial protein, Tir (translocated intimin receptor), which is exported by the bacteria and integrated into the host cell plasma membrane. Importantly, EPEC (O127:H6) and EHEC (O157:H7) express antigenically distinct intimin types known as intimin alpha and gamma, respectively. EHEC (O157:H7) colonises human intestinal explants although adhesion is restricted to the follicle associated epithelium of Peyer's patches. This phenotype is also observed with EPEC O127:H6 engineered to express EHEC intimin gamma.
aimsTo investigate the influence of intimin on colonisation of human intestine by E coli O157:H7, and intimin types on tissue tropism in humans.
methodsHuman intestinal in vitro organ culture with wild type and mutant strains of O157:H7 were employed.
resultsIntroducing a deletion mutation in the eae gene encoding intimin gamma in EHEC (O157:H7) caused the strain (ICC170) to fail to colonise human intestinal explants. However, colonisation of Peyer's patches and A/E lesion formation were restored with intimin gamma expression from a plasmid (ICC170 (pICC55)). In contrast, complementing the mutation with intimin alpha resulted in a strain (ICC170 (pCVD438)) capable of colonising and producing A/E lesions on both Peyer's patch and other small intestinal explants.
conclusionIntimin is necessary for human intestinal mucosal colonisation by E coli O157:H7. Intimin type influences the site of colonisation in a Tir type independent mechanism; intimin gamma appears to restrict colonisation to human follicle associated epithelium.
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