ArticleJournal of neuroscience research2002
Constitutive overexpression of the basic helix-loop-helix Nex1/MATH-2 transcription factor promotes neuronal differentiation of PC12 cells and neurite regeneration.
Article in Journal of neuroscience research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 61 citations in OpenAlex.
- Article
- Enhancing structural plasticity of PC12 neurons during differentiation and neurite regeneration with a catalytically inactive mutant version of the zRICH protein.BMC neuroscience · 2023Article
- A quantitative evaluation of topological motifs and their coupling in gene circuit state distributions.iScience · 2023Article
- βIII-Tubulin Gene Regulation in Health and Disease.Frontiers in cell and developmental biology · 2022Review
- The Role of Neurod Genes in Brain Development, Function, and Disease.Frontiers in molecular neuroscience · 2021Article
- Epigenetic modifiers promote mitochondrial biogenesis and oxidative metabolism leading to enhanced differentiation of neuroprogenitor cells.Cell death & disease · 2018Article
- Novel subcellular localization of the DNA helicase Twinkle at the kinetochore complex during mitosis in neuronal-like progenitor cells.Histochemistry and cell biology · 2016Article
- Mitochondrial biogenesis: a therapeutic target for neurodevelopmental disorders and neurodegenerative diseases.Current pharmaceutical design · 2014Review
- RNA-Seq data mining: downregulation of NeuroD6 serves as a possible biomarker for alzheimer's disease brains.Disease markers · 2014Article
- The neurogenic basic helix-loop-helix transcription factor NeuroD6 enhances mitochondrial biogenesis and bioenergetics to confer tolerance of neuronal PC12-NeuroD6 cells to the mitochondrial stressor rotenone.Experimental cell research · 2012Article
- PRG-1 transcriptional regulation independent from Nex1/Math2-mediated activation.Cellular and molecular life sciences : CMLS · 2012Article
- Article
- NeuroD6 genomic signature bridging neuronal differentiation to survival via the molecular chaperone network.Journal of neuroscience research · 2010Article
- Cloning and characterization of the 5'UTR of the rat anti-apoptotic Bcl-w gene.Biochemical and biophysical research communications · 2009Article
- Article
- YB-1 transcription in the postnatal brain is regulated by a bHLH transcription factor Math2 through an E-box sequence in the 5'-UTR of the gene.Molecular and cellular biochemistry · 2009Article
- A high throughput embryonic stem cell screen identifies Oct-2 as a bifunctional regulator of neuronal differentiation.Genes & development · 2009Article
- A transgenic mouse class-III beta tubulin reporter using yellow fluorescent protein.Genesis (New York, N.Y. : 2000) · 2007Article
- 5'UTR of the neurogenic bHLH Nex1/MATH-2/NeuroD6 gene is regulated by two distinct promoters through CRE and C/EBP binding sites.Journal of neuroscience research · 2007Article
- The basic helix-loop-helix transcription factor Nex-1/Math-2 promotes neuronal survival of PC12 cells by modulating the dynamic expression of anti-apoptotic and cell cycle regulators.Journal of neurochemistry · 2005Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Elucidation of the intricate transcriptional pathways leading to neural differentiation and the establishment of neuronal identity is critical to the understanding and design of therapeutic approaches. Among the important players, the basic helix-loop-helix (bHLH) transcription factors have been found to be pivotal regulators of neurogenesis. In this study, we investigate the role of the bHLH differentiation factor Nex1/MATH-2 in conjunction with the nerve growth factor (NGF) signaling pathway using the rat phenochromocytoma PC12 cell line. We report that the expression of Nex1 protein is induced after 5 hr of NGF treatment and reaches maximal levels at 24 hr, when very few PC12 cells have begun extending neurites and ceased cell division. Furthermore, our study demonstrates that Nex1 has the ability to trigger neuronal differentiation of PC12 cells in the absence of neurotrophic factor. We show that Nex1 plays an important role in neurite outgrowth and has the capacity to regenerate neurite outgrowth in the absence of NGF. These results are corroborated by the fact that Nex1 targets a repertoire of distinct types of genes associated with neuronal differentiation, such as GAP-43, betaIII-tubulin, and NeuroD. In addition, our findings show that Nex1 up-regulates the expression of the mitotic inhibitor p21(WAF1), thus linking neuronal differentiation to cell cycle withdrawal. Finally, our studies show that overexpression of a Nex1 mutant has the ability to block the execution of NGF-induced differentiation program, suggesting that Nex1 may be an important effector of the NGF signaling pathway.
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