ArticleProceedings of the National Academy of Sciences of the United States of America2002
The large GTPase dynamin regulates actin comet formation and movement in living cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 98 papers.
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Who cites it
98 citing papers in PubMed, 225 citations in OpenAlex.
- High-fat diet-responsive DNM1 promotes hepatocellular carcinoma progression and predicts poor prognosis in viral-associated patients.American journal of cancer research · 2026Article
- Persistence of quantal synaptic vesicle recycling in virtual absence of dynamins.The Journal of physiology · 2025Article
- Endolysosomal trafficking controls yolk granule biogenesis in vitellogenic Drosophila oocytes.PLoS genetics · 2024Article
- Cell entry of bovine respiratory syncytial virus through clathrin-mediated endocytosis is regulated by PI3K-Akt and Src-JNK pathways.Frontiers in microbiology · 2024Article
- Schisandra chinensis inhibits the entry of BoHV-1 by blocking PI3K-Akt pathway and enhances the m6A methylation of gD to inhibit the entry of progeny virus.Frontiers in microbiology · 2024Article
- Palmitate-mediated disruption of the endoplasmic reticulum decreases intracellular vesicle motility.Biophysical journal · 2023Article
- Seneca Valley Virus Enters PK-15 Cells via Caveolae-Mediated Endocytosis and Macropinocytosis Dependent on Low-pH, Dynamin, Rab5, and Rab7.Journal of virology · 2022Article
- The molecular organization of differentially curved caveolae indicates bendable structural units at the plasma membrane.Nature communications · 2022Article
- GDAP1 loss of function inhibits the mitochondrial pyruvate dehydrogenase complex by altering the actin cytoskeleton.Communications biology · 2022Article
- Porcine Deltacoronavirus Enters Porcine IPI-2I Intestinal Epithelial Cells via Macropinocytosis and Clathrin-Mediated Endocytosis Dependent on pH and Dynamin.Journal of virology · 2021Article
- Distinct forms of the actin cross-linking protein α-actinin support macropinosome internalization and trafficking.Molecular biology of the cell · 2021Article
- Scavenger receptor MARCO contributes to cellular internalization of exosomes by dynamin-dependent endocytosis and macropinocytosis.Scientific reports · 2020Article
- Hsv-1 Endocytic Entry into a Human Oligodendrocytic Cell Line is Mediated by Clathrin and Dynamin but Not Caveolin.Viruses · 2020Article
- Dynamin regulates the dynamics and mechanical strength of the actin cytoskeleton as a multifilament actin-bundling protein.Nature cell biology · 2020Article
- Inside(sight) of tiny communicator: exosome biogenesis, secretion, and uptake.Molecular and cellular biochemistry · 2020Review
- GGA3-mediated recycling of the RET receptor tyrosine kinase contributes to cell migration and invasion.Oncogene · 2020Article
- Porous Silicon Nanoneedles Modulate Endocytosis to Deliver Biological Payloads.Advanced materials (Deerfield Beach, Fla.) · 2019Article
- Dynamin Autonomously Regulates Podocyte Focal Adhesion Maturation.Journal of the American Society of Nephrology : JASN · 2017Article
- Functional Role of Gonadotrope Plasticity and Network Organization.Frontiers in endocrinology · 2017Review
- Actin filaments as dynamic reservoirs for Drp1 recruitment.Molecular biology of the cell · 2016Article
38 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The large GTPase dynamin (Dyn2) has been demonstrated by us and others to interact with several different actin-binding proteins. To define how Dyn2 might participate in actin dynamics in livings cells we have expressed green fluorescent protein (GFP)-tagged Dyn2 in cultured cells and observed labeling of comet-like vesicles and macropinosomes. The comet structures progressed with a constant velocity and were reminiscent of actin comets associated with motile vesicles in cells expressing type I phosphatidylinositol phosphate 5-kinases. Based on these observations we sought to determine whether Dyn2 is an integral component of actin comets. Cells expressing type I phosphatidylinositol phosphate 5-kinase and Dyn2-GFP revealed a prominent colocalization of Dyn2 and actin in comet structures. Interestingly, comet formation and motility were normal in cells expressing wild-type Dyn2-GFP but altered markedly in Dyn2 mutant-expressing cells. Dyn2K44A-GFP mutant cells displayed a significant reduction in comet number, length, velocity, and efficiency of movement. In contrast, comets in cells expressing Dyn2DeltaPRD-GFP appeared dark and did not incorporate the mutant Dyn2 protein, indicating that the proline-rich domain (PRD) is required for Dyn2 recruitment. Further, these comets were significantly longer and slower than those in control cells. These findings demonstrate a role for Dyn2 in actin-based vesicle motility.
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