ArticleProceedings of the National Academy of Sciences of the United States of America2002
Suppressors of transcriptional transgenic silencing in Chlamydomonas are sensitive to DNA-damaging agents and reactivate transposable elements.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 95 citations in OpenAlex.
- Increasing lipid production inFrontiers in bioengineering and biotechnology · 2024Article
- Small RNAs >26 nt in length associate with AGO1 and are upregulated by nutrient deprivation in the alga Chlamydomonas.The Plant cell · 2023Article
- Rates and spectra of de novo structural mutations inGenome research · 2023Article
- Improved tolerance of recombinant Chlamydomonas rainhardtii with putative 2-amino-3-carboxymuconate-6-semialdehyde decarboxylase from Pyropia yezoensis to nitrogen starvation.Journal of microbiology (Seoul, Korea) · 2022Article
- The epigenetic origin of life history transitions in plants and algae.Plant reproduction · 2021Review
- Development and characterization of aBiotechnology for biofuels · 2020Article
- Inhibition of DNA Methylation inFrontiers in genetics · 2020Article
- Good News for Nuclear Transgene Expression inCells · 2019Review
- Complementation of a mutation in CpSRP43 causing partial truncation of light-harvesting chlorophyll antenna in Chlorella vulgaris.Scientific reports · 2017Article
- Impacts of elevated COScientific reports · 2017Article
- Current status and perspectives of genome editing technology for microalgae.Biotechnology for biofuels · 2017Review
- CRISPR/Cas9-induced knockout and knock-in mutations in Chlamydomonas reinhardtii.Scientific reports · 2016Article
- Heterologous overexpression of sfCherry fluorescent protein inBiotechnology reports (Amsterdam, Netherlands) · 2015Article
- Osmotic stress induces phosphorylation of histone H3 at threonine 3 in pericentromeric regions of Arabidopsis thaliana.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Effects of overexpression of a bHLH transcription factor on biomass and lipid production in Nannochloropsis salina.Biotechnology for biofuels · 2015Article
- Using natural selection to explore the adaptive potential of Chlamydomonas reinhardtii.PloS one · 2014Article
- Biofuels from algae: challenges and potential.Biofuels · 2010Article
- Unstable RNAi effects through epigenetic silencing of an inverted repeat transgene in Chlamydomonas reinhardtii.Genetics · 2008Article
- Diversification of the core RNA interference machinery in Chlamydomonas reinhardtii and the role of DCL1 in transposon silencing.Genetics · 2008Article
- The MUT9p kinase phosphorylates histone H3 threonine 3 and is necessary for heritable epigenetic silencing in Chlamydomonas.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the unicellular green alga Chlamydomonas reinhardtii, the epigenetic silencing of transgenes occurs, as in land plants, at both the transcriptional and posttranscriptional levels. In the case of single-copy transgenes, transcriptional silencing takes place without detectable cytosine methylation of the introduced DNA. We have isolated two mutant strains, Mut-9 and Mut-11, that reactivate expression of a transcriptionally silenced single-copy transgene. These suppressors are deficient in the repression of a DNA transposon and a retrotransposon-like element. In addition, the mutants show enhanced sensitivity to DNA-damaging agents, particularly radiomimetic chemicals inducing DNA double-strand breaks. All of these phenotypes are much more prominent in a double mutant strain. These observations suggest that multiple partly redundant epigenetic mechanisms are involved in the repression of transgenes and transposons in eukaryotes, presumably as components of a system that evolved to preserve genomic stability. Our results also raise the possibility of mechanistic connections between epigenetic transcriptional silencing and DNA double-strand break repair.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.