ArticleGenes & development2002
The KH-type RNA-binding protein PSI is required for Drosophila viability, male fertility, and cellular mRNA processing.
Article in Genes & development, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 37 citations in OpenAlex.
- Psi promotes Drosophila wing growth via direct transcriptional activation of cell cycle targets and repression of growth inhibitors.Development (Cambridge, England) · 2023Article
- Article
- P-element Somatic Inhibitor Protein Binding a Target Sequence inInternational journal of molecular sciences · 2019Article
- A Robust Transposon-Endogenizing Response from Germline Stem Cells.Developmental cell · 2018Article
- JUM is a computational method for comprehensive annotation-free analysis of alternative pre-mRNA splicing patterns.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Bombyx mori P-element Somatic Inhibitor (BmPSI) Is a Key Auxiliary Factor for Silkworm Male Sex Determination.PLoS genetics · 2017Article
- The PSI-U1 snRNP interaction regulates male mating behavior in Drosophila.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- P Transposable Elements in Drosophila and other Eukaryotic Organisms.Microbiology spectrum · 2015Review
- Mechanisms and Regulation of Alternative Pre-mRNA Splicing.Annual review of biochemistry · 2015Review
- The Drosophila splicing factor PSI is phosphorylated by casein kinase II and tousled-like kinase.PloS one · 2013Article
- Long-term and short-term evolutionary impacts of transposable elements on Drosophila.Genetics · 2012Article
- ZBP2 facilitates binding of ZBP1 to beta-actin mRNA during transcription.Molecular and cellular biology · 2007Article
- The SR family proteins B52 and dASF/SF2 modulate development of the Drosophila visual system by regulating specific RNA targets.Molecular and cellular biology · 2007Article
- Distinct contributions of KH domains to substrate binding affinity of Drosophila P-element somatic inhibitor protein.RNA (New York, N.Y.) · 2006Article
- Global analysis of positive and negative pre-mRNA splicing regulators in Drosophila.Genes & development · 2005Article
- Differentiation-induced colocalization of the KH-type splicing regulatory protein with polypyrimidine tract binding protein and the c-src pre-mRNA.Molecular biology of the cell · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Direct interactions between RNA-binding proteins and snRNP particles modulate eukaryotic pre-mRNA processing patterns to control gene expression. Here, we report that the conserved U1 snRNP-interacting RNA-binding protein PSI is essential for Drosophila viability. A null PSI mutation is recessive lethal at the first-instar larval stage, and lethality is fully rescued by transgenes expressing the PSI protein. A mutant transgene that lacks the PSI-U1 snRNP-interaction domain restores viability but shows courtship behavior abnormalities and meiosis defects during spermatogenesis, resulting in a complete male sterility phenotype. Using cDNA microarrays, we have identified specific target mRNAs with altered expression profiles in these mutant males. A subset of these transcripts is also found associated with PSI in endogenous immunopurified ribonucleoprotein complexes. One specific target, the hrp40/squid transcript, shows an altered pre-mRNA splicing pattern in PSI mutant testes. We conclude that a functional association between the PSI protein and the spliceosomal U1 snRNP particle is required for normal Drosophila development and for the processing of specific PSI-interacting cellular transcripts. These results also validate the use of cDNA microarrays to characterize in vivo RNA-processing defects and alternative pre-mRNA splicing patterns.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.