ArticleGenes & development2002
PPARgamma knockdown by engineered transcription factors: exogenous PPARgamma2 but not PPARgamma1 reactivates adipogenesis.
Article in Genes & development, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 114 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
114 citing papers in PubMed, 354 citations in OpenAlex.
- Co-Administration of Vitamin E and Atorvastatin Improves Insulin Sensitivity and Peroxisome Proliferator-Activated Receptor-γ Expression in Type 2 Diabetic Patients: A Randomized Double-Blind Clinical Trial.Iranian journal of medical sciences · 2022Trial
- Adipose Tissue Plasticity and Adipogenesis: From Cellular Mechanisms to Therapeutic Targets in Obesity.Current obesity reports · 2026Review
- Role of adipose tissue in cardiovascular health and diseases.Chinese medical journal · 2026Review
- Extensive enhancer crosstalk controls PPARG2 activation during adipogenesis.Nature communications · 2026Article
- Deregulated miR-145 and miR-27b in Hutchinson-Gilford progeria syndrome: implications for adipogenesis.Aging · 2025Article
- STIM2β is a CaThe FEBS journal · 2025Article
- White Adipocyte Stem Cell Expansion Through Infant Formula Feeding: New Insights into Epigenetic Programming Explaining the Early Protein Hypothesis of Obesity.International journal of molecular sciences · 2025Review
- Review
- Limited oxygen in standard cell culture alters metabolism and function of differentiated cells.The EMBO journal · 2024Article
- Immunogenetic Aspects of Sarcopenic Obesity.Genes · 2024Review
- Depot-specific mRNA expression programs in human adipocytes suggest physiological specialization via distinct developmental programs.PloS one · 2024Article
- Familial partial lipodystrophy resulting from loss-of-function PPARγ pathogenic variants: phenotypic, clinical, and genetic features.Frontiers in endocrinology · 2024Review
- Article
- Growth and adiposity in newborns study (GAINS): The influence of prenatal DHA supplementation protocol.Contemporary clinical trials · 2023Article
- Cell fate determining molecular switches and signaling pathways in Pax7-expressing somitic mesoderm.Cell discovery · 2022Article
- The circadian clock has roles in mesenchymal stem cell fate decision.Stem cell research & therapy · 2022Review
- Perspectives on scaling production of adipose tissue for food applications.Biomaterials · 2022Article
- The joint effect of PPARG upstream genetic variation in association with long-term persistent obesity: Tehran cardio-metabolic genetic study (TCGS).Eating and weight disorders : EWD · 2021Article
- PPARs-Orchestrated Metabolic Homeostasis in the Adipose Tissue.International journal of molecular sciences · 2021Review
- Transcriptional networks controlling stromal cell differentiation.Nature reviews. Molecular cell biology · 2021Review
54 more citing papers are in PubMed but not listed here.
Corrections and comments
- Commented on byBecoming fat.2002
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To determine functional differences between the two splice variants of PPARgamma (gamma1 and gamma2), we sought to selectively repress gamma2 expression by targeting engineered zinc finger repressor proteins (ZFPs) to the gamma2-specific promoter, P2. In 3T3-L1 cells, expression of ZFP55 resulted in >50% reduction in gamma2 expression but had no effect on gamma1, whereas adipogenesis was similarly reduced by 50%. However, ZFP54 virtually abolished both gamma2 and gamma1 expression, and completely blocked adipogenesis. Overexpression of exogenous gamma2 in the ZFP54-expressing cells completely restored adipogenesis, whereas overexpression of gamma1 had no effect. This finding clearly identifies a unique role for the PPARgamma2 isoform.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.