ArticleRNA (New York, N.Y.)2001
A conserved role of a DEAD box helicase in mRNA masking.
Article in RNA (New York, N.Y.), 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
71 citing papers in PubMed, 143 citations in OpenAlex.
- Deadenylation and decapping factors cooperatively stimulate biochemical activities of DEAD-box ATPase Dhh1.Nucleic acids research · 2026Article
- Deadenylation and Decapping Factors Cooperatively Stimulate Biochemical Activities of DEAD-Box ATPase Dhh1.bioRxiv : the preprint server for biology · 2026Article
- Me31B: a key repressor in germline regulation and beyond.Bioscience reports · 2024Review
- P-body-like condensates in the germline.Seminars in cell & developmental biology · 2024Review
- An essential role for the RNA helicase DDX6 in NMDA receptor-dependent gene silencing and dendritic spine shrinkage.Scientific reports · 2024Article
- Mutational analysis of the functional motifs of the DEAD-box RNA helicase Me31B/DDX6 in Drosophila germline development.FEBS letters · 2023Article
- Tethered Function Assays to Elucidate the Role of RNA-Binding Proteins.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Tyramine induces dynamic RNP granule remodeling and translation activation in the Drosophila brain.eLife · 2021Article
- Musashi interaction with poly(A)-binding protein is required for activation of target mRNA translation.The Journal of biological chemistry · 2019Article
- Translational repression of theRNA (New York, N.Y.) · 2017Article
- Article
- Cellular DEAD-box RNA helicase DDX6 modulates interaction of miR-122 with the 5' untranslated region of hepatitis C virus RNA.Virology · 2017Article
- Binding of DEAD-box helicase Dhh1 to the 5'-untranslated region ofThe Journal of biological chemistry · 2017Article
- Insertion of inter-domain linkers improves expression and bioactivity of Zygote arrest (Zar) fusion proteins.Protein engineering, design & selection : PEDS · 2017Article
- Dual mechanisms regulate the nucleocytoplasmic localization of human DDX6.Scientific reports · 2017Article
- Article
- Controlling the Messenger: Regulated Translation of Maternal mRNAs in Xenopus laevis Development.Advances in experimental medicine and biology · 2017Review
- Zebrafish P54 RNA helicases are cytoplasmic granule residents that are required for development and stress resilience.Biology open · 2016Article
- The DDX6-4E-T interaction mediates translational repression and P-body assembly.Nucleic acids research · 2016Article
- The WEE1 regulators CPEB1 and miR-15b switch from inhibitor to activators at G2/M.Cell cycle (Georgetown, Tex.) · 2016Article
11 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clam p82 is a member of the cytoplasmic polyadenylation element-binding protein (CPEB) family of RNA-binding proteins and serves dual functions in regulating gene expression in early development. In the oocyte, p82/CPEB is a translational repressor, whereas in the activated egg, it acts as a polyadenylation factor. Coimmunoprecipitations were performed with p82 antibodies in clam oocyte and egg lysates to identify stage-regulated accessory factors. p47 coprecipitates with p82 from oocyte lysates in an RNA-dependent manner and is absent from egg lysate p92-bound material. Clam p47 is a member of the RCK/p54 family of DEAD box RNA helicases. Xp54, the Xenopus homolog, with bona fide helicase activity, is an abundant and integral component of stored mRNP in oocytes (Ladomery et al., 1997). In oocytes, clam p47 and p82/CPEB are found in large cytoplasmic mRNP complexes. Whereas the helicase level is constant during embryogenesis, in contrast to CPEB, clam p47 translocates to nuclei at the two-cell stage. To address the role of this class of helicase in masking, Xp54 was tethered via 3' UTR MS2-binding sites to firefly luciferase, following microinjection of fusion protein and nonadenylated reporter mRNAs into Xenopus oocytes. Tethered helicase repressed luciferase translation three- to fivefold and, strikingly, mutations in two helicase motifs (DEAD--> DQAD and HRIGR-->HRIGQ), activated translation three- to fourfold, relative to MS2. These data suggest that this helicase family represses translation of maternal mRNA in early development, and that its activity may be attenuated during meiotic maturation, prior to cytoplasmic polyadenylation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.