ArticleCellular and molecular neurobiology2001
Establishment and partial characterization of a continuous human malignant glioma cell line: NG97.
Article in Cellular and molecular neurobiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Spider venom components decrease glioblastoma cell migration and invasion through RhoA-ROCK and NaCancer cell international · 2020Article
- Establishment and partial characterization of a human tumor cell line, GBM-HSF, from a glioblastoma multiforme.Human cell · 2014Article
- Galectin-3 up-regulation in hypoxic and nutrient deprived microenvironments promotes cell survival.PloS one · 2014Article
- In vivo models of primary brain tumors: pitfalls and perspectives.Neuro-oncology · 2012Review
- Characterization of cells recovered from the xenotransplanted NG97 human-derived glioma cell line subcultured in a long-term in vitro.BMC cancer · 2008Article
- Establishment of a new human glioblastoma multiforme cell line (WJ1) and its partial characterization.Cellular and molecular neurobiology · 2007Article
- Immunophenotypic and ultrastructural validation of a new human glioblastoma cell line.Cellular and molecular neurobiology · 2005Article
- Morphological characterization of a human glioma cell l ine.Cancer cell international · 2005Article
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Authors and funding
5 authors.
Funding
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Abstract
1. A human glioma cell line, NG97, was established from tissue obtained from a patient diagnosed with a grade III astrocytoma. 2. The NG97 cell line has been subcultured for more than 100 passages in standard culture media without feeder layer or collagen coatings. 3. NG97 cells grow in vitro as two subpopulations with distinct morphological appearance: stellate cells with pleomorphic nuclei, and small round cells with few processes. The cells have a doubling time of about 72 h and a plating efficiency of 1%. The injection of NG97 cells into congenitally athymic mice induced the formation of solid tumor masses that could be retransplanted every 4 weeks. The cells obtained from tumor mass when cultivated in vitro had a morphology comparable to those of the initial culture. 4. This cell line may prove useful for cellular and molecular studies as well as in studies of gliomas treatment.
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