ArticleCellular and molecular neurobiology2001
Cell density-dependent death mode switch of cultured cortical neurons under serum-free starvation stress.
Article in Cellular and molecular neurobiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 30 citations in OpenAlex.
- Effect of prothymosin α on neuroplasticity following cerebral ischemia‑reperfusion injury.Molecular medicine reports · 2024Article
- Hydroxyobtustyrene protects neuronal cells from chemical hypoxia-induced cell death.Journal of natural medicines · 2018Article
- The calmodulin-dependent protein kinase II inhibitor KN-93 protects rat cerebral cortical neurons from N-methyl-D-aspartic acid-induced injury.Neural regeneration research · 2013Article
- Long-term primary culture of highly-pure rat embryonic hippocampal neurons of low-density.Neurochemical research · 2010Article
- Prothymosin alpha plays a key role in cell death mode-switch, a new concept for neuroprotective mechanisms in stroke.Naunyn-Schmiedeberg's archives of pharmacology · 2008Review
- Increased cell suspension concentration augments the survival rate of grafted tyrosine hydroxylase immunoreactive neurons.Journal of neuroscience methods · 2007Article
- Identification of prothymosin-alpha1, the necrosis-apoptosis switch molecule in cortical neuronal cultures.The Journal of cell biology · 2007Article
- A serum- and antioxidant-free primary culture model of mouse cortical neurons for pharmacological screen and studies of neurotrophic and neuroprotective agents.Cellular and molecular neurobiology · 2002Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. Cell death mode switch of cortical neurons from E17 rats was studied. Cells rapidly died under the serum-free condition. The time-course of cell death was markedly delayed by increasing cell density for primary culture in the trypan blue exclusion, LDH release, and MTT assays. 2. By analyzing cell death by the use of double staining using PI/TUNEL and PI/Annexin V combinations, the mode in the low density culture was found to be necrosis, while that in the high density culture was apoptosis. 3. The intracellular ATP level after the start of serum-free culture rapidly decline to 25% of 0-time level in the low density culture, but it was 60% in the high density culture. Both oligomycin and zVAD-fmk markedly decreased ATP levels and the population of TUNEL-positive neurons, while 3-aminobenzamide slightly increased these indices. 4. Thus. it is strongly suggested that the cell death mode switch from necrosis to apoptosis is closely related to intracellular ATP levels, and some conditioned medium factors observed in the high density culture may affect both ATP level and cell death mode switch.
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