ArticleProceedings of the National Academy of Sciences of the United States of America2002
Insect NMDA receptors mediate juvenile hormone biosynthesis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 103 citations in OpenAlex.
- Innovative Technologies for Kidney Research: Three-Dimensional Imaging and Quantification.Seminars in nephrology · 2026Review
- A Peptidergic Neural System Connects the Circadian Clock to the Photoperiodic Control of Reproductive Diapause in the BugThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Article
- The PBAP chromatin remodeling complex mediates summer diapause via H3K4me3-driven juvenile hormone regulation inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- Article
- The Role of SCL Isoforms in Embryonic Hematopoiesis.International journal of molecular sciences · 2023Article
- NMDA Receptor and Its Emerging Role in Cancer.International journal of molecular sciences · 2023Review
- Article
- Clock gene-dependent glutamate dynamics in the bean bug brain regulate photoperiodic reproduction.PLoS biology · 2022Article
- Single-Step Fast Tissue Clearing of Thick Mouse Brain Tissue for Multi-Dimensional High-Resolution Imaging.International journal of molecular sciences · 2022Article
- Juvenile Hormone Studies inFrontiers in physiology · 2021Review
- Regulation ofFrontiers in neuroscience · 2021Article
- Clearing techniques for visualizing the nervous system in development, injury, and disease.Journal of neuroscience methods · 2020Review
- Neural shutdown under stress: an evolutionary perspective on spreading depolarization.Journal of neurophysiology · 2020Review
- Dissecting Neuronal Activation on a Brain-Wide Scale With Immediate Early Genes.Frontiers in neuroscience · 2020Review
- Routine Optical Clearing of 3D-Cell Cultures: Simplicity Forward.Frontiers in molecular biosciences · 2020Article
- Comparative Transcriptome Analysis for Understanding Predator-Induced Polyphenism in the Water FleaInternational journal of molecular sciences · 2018Article
- Three-Dimensional Analysis of the Human Pancreas.Endocrinology · 2018Article
- Central neural alterations predominate in an insect model of nociceptive sensitization.The Journal of comparative neurology · 2017Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In vertebrates, the N-methyl-D-aspartate subtype of glutamate receptors (NMDAR) appears to play a role in neuronal development, synaptic plasticity, memory formation, and pituitary activity. However, functional NMDAR have not yet been characterized in insects. We have now demonstrated immunohistochemically glutamatergic nerve terminals in the corpora allata of an adult female cockroach, Diploptera punctata. Cockroach corpus allatum (CA) cells, exposed to NMDA in vitro, exhibited elevated cytosolic [Ca(2+)], but not in culture medium nominally free of calcium or containing NMDAR-specific channel blockers: MK-801 and Mg(2+). Sensitivity of cockroach corpora allata to NMDA changed cyclically during the ovarian cycle. Highly active glands of 4-day-old mated females, exposed to 3 microM NMDA, produced 70% more juvenile hormone (JH) in vitro, but the relatively inactive glands of 8-day-old mated females showed little response to the agonist. The stimulatory effect of NMDA was eliminated by augmenting the culture medium with MK-801, conantokin, or high Mg(2+). Having obtained substantive evidence of functioning NMDAR in insect corpora allata, we used reverse transcription PCR to demonstrate two mRNA transcripts, DNMDAR1 and DNMDAR2, in the ring gland and brain of last-instar Drosophila melanogaster. Immunohistochemical labeling, using mouse monoclonal antibody against rat NMDAR1, showed that only one of the three types of endocrine cells in the ring gland, CA cells, expressed rat NMDAR1-like immunoreactive protein. This antibody also labeled two brain neurons in the lateral protocerebrum, one neuron per brain hemisphere. Finally, we used the same primers for DNMDAR1 to demonstrate a fragment of putative NMDA receptor in the corpora allata of Diploptera punctata. Our results suggest that the NMDAR has a role in regulating JH synthesis and that ionotropic-subtype glutamate receptors became specialized early in animal evolution.
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