ArticleJournal of virology2002
Kissing interaction between 3' noncoding and coding sequences is essential for porcine arterivirus RNA replication.
Article in Journal of virology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
40 citing papers in PubMed, 65 citations in OpenAlex.
- Ten years after introduction of NADC30-like strain in China: a novel chimeric porcine reproductive and respiratory syndrome vaccine candidate.Frontiers in immunology · 2025Article
- Development and biological characterization of an infectious cDNA clone of NADC34-like PRRSV.Frontiers in microbiology · 2024Article
- The 3' end of the coding region of senecavirus A contains a highly conserved sequence that potentially forms a stem-loop structure required for virus rescue.Archives of virology · 2023Article
- Article
- Classification, replication, and transcription ofFrontiers in microbiology · 2023Review
- Article
- Porcine Reproductive and Respiratory Syndrome Virus Reverse Genetics and the Major Applications.Viruses · 2020Review
- Structures and Functions of the 3' Untranslated Regions of Positive-Sense Single-Stranded RNA Viruses Infecting Humans and Animals.Frontiers in cellular and infection microbiology · 2020Review
- A Polymer Physics Framework for the Entropy of Arbitrary Pseudoknots.Biophysical journal · 2019Article
- The Nsp12-coding region of type 2 PRRSV is required for viral subgenomic mRNA synthesis.Emerging microbes & infections · 2019Article
- Article
- Identification of the RNA Pseudoknot within the 3' End of the Porcine Reproductive and Respiratory Syndrome Virus Genome as a Pathogen-Associated Molecular Pattern To Activate Antiviral Signaling via RIG-I and Toll-Like Receptor 3.Journal of virology · 2018Article
- Characterization of two Austrian porcine reproductive and respiratory syndrome virus (PRRSV) field isolates reveals relationship to East Asian strains.Veterinary research · 2016Article
- Inhibition of porcine reproductive and respiratory syndrome virus replication in vitro using DNA-based short antisense oligonucleotides.BMC veterinary research · 2015Article
- PRRSV structure, replication and recombination: Origin of phenotype and genotype diversity.Virology · 2015Review
- Mapping overlapping functional elements embedded within the protein-coding regions of RNA viruses.Nucleic acids research · 2014Article
- Attenuation and immunogenicity of a live high pathogenic PRRSV vaccine candidate with a 32-amino acid deletion in the nsp2 protein.Journal of immunology research · 2014Article
- A novel isolate with deletion in GP3 gene of porcine reproductive and respiratory syndrome virus from mid-eastern China.BioMed research international · 2014Article
- Disulfide linkages mediating nucleocapsid protein dimerization are not required for porcine arterivirus infectivity.Journal of virology · 2012Article
- Cis-acting structural element in 5' UTR is essential for infectivity of porcine reproductive and respiratory syndrome virus.Virus research · 2012Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We used an infectious cDNA clone of porcine reproductive and respiratory syndrome virus (PRRSV) to investigate the presence of essential replication elements in the region of the genome encoding the structural proteins. Deletion analysis showed that a stretch of 34 nucleotides (14653 to 14686) within ORF7, which encodes the nucleocapsid protein, is essential for RNA replication. Strand-specific reverse transcription-PCR analysis of viral RNA isolated from transfected BHK-21 cells revealed that this region is required for negative-strand genomic RNA synthesis. The 34-nucleotide stretch is highly conserved among PRRSV isolates and folds into a putative hairpin. A 7-base sequence within the loop of this structure was suggested to base-pair with a sequence present in the loop of a hairpin located in the 3' noncoding region, resulting in a kissing interaction. Mutational analyses confirmed that this kissing interaction is required for RNA replication.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.