ArticleJournal of clinical microbiology2002
Serotype identification of group B streptococci by PCR and sequencing.
Article in Journal of clinical microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
74 citing papers in PubMed, 1 synthesis or guideline pooled it, 150 citations in OpenAlex.
- One hypervirulent clone, sequence type 283, accounts for a large proportion of invasive Streptococcus agalactiae isolated from humans and diseased tilapia in Southeast Asia.PLoS neglected tropical diseases · 2019Pooled it
- Invasive Streptococcus agalactiae infections in infants in Guangzhou, Southern China (2013-2022): molecular epidemiology and clinical management implications.BMC microbiology · 2026Article
- Towards a holistic epidemiology ofFrontiers in bioinformatics · 2026Article
- Group B streptococcal infections in pregnancy and early life.Clinical microbiology reviews · 2025Review
- Geographical, Temporal and Host-Species Distribution of Potentially Human-Pathogenic Group BPathogens (Basel, Switzerland) · 2023Article
- Genotype Distribution and Antibiotic Susceptibility Pattern of Clinical Isolates of Group BJournal of tropical medicine · 2023Article
- Clinical and molecular epidemiology of invasive group B Streptococcus infections in adults in a referral center in Korea.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2022Article
- Epidemiology and bacterial characteristics of invasive group B streptococcus disease: a population-based study in Japan in 2010-2020.Epidemiology and infection · 2022Article
- GBS-SBG - GBS Serotyping by Genome Sequencing.Microbial genomics · 2021Article
- A Narrative Review of the Molecular Epidemiology and Laboratory Surveillance of Vaccine Preventable Bacterial Meningitis Agents:Microorganisms · 2021Review
- Molecular Characterization ofJournal of tropical medicine · 2020Article
- Microevolution of Streptococcus agalactiae ST-261 from Australia Indicates Dissemination via Imported Tilapia and Ongoing Adaptation to Marine Hosts or Environment.Applied and environmental microbiology · 2018Article
- Review
- Identification of Group B Streptococcus Capsule Type by Use of a Dual Phenotypic/Genotypic Assay.Journal of clinical microbiology · 2017Article
- Comparison of molecular serotyping approaches of Streptococcus agalactiae from genomic sequences.BMC genomics · 2017Article
- Molecular Characterization of Streptococcus agalactiae Isolates From Pregnant and Non-Pregnant Women at Yazd University Hospital, Iran.Jundishapur journal of microbiology · 2016Article
- Analysis of Streptococcus agalactiae pan-genome for prevalence, diversity and functionality of integrative and conjugative or mobilizable elements integrated in the tRNA(Lys CTT) gene.Molecular genetics and genomics : MGG · 2015Article
- Group B Streptococcus vaccine: state of the art.Therapeutic advances in vaccines · 2015Review
- Association between capsular serotype V and macrolide resistance in group B Streptococcus.Epidemiology and infection · 2015Article
- Survey of immunological features of the alpha-like proteins of Streptococcus agalactiae.Clinical and vaccine immunology : CVI · 2015Review
14 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Group B streptococcus (GBS; Streptococcus agalactiae) is the most common cause of neonatal and obstetric sepsis and is an increasingly important cause of septicemia in elderly individuals and immunocompromised patients. Ongoing surveillance to monitor GBS serotype distribution will be needed to guide the development and use of GBS conjugate vaccines. We designed sequencing primers based on the previously published sequences of the capsular polysaccharide (cps) gene clusters to further define partial cps gene clusters for eight of the nine GBS serotypes (serotypes Ia to VII). Subsequently, we designed and evaluated primers to identify serotypes Ia, Ib, III, IV, V, and VI directly by PCR and all eight serotypes (serotypes Ia to VII) by sequence heterogeneity. A total of 206 clinical GBS isolates were used to compare our molecular serotype (MS) identification method with conventional serotyping (CS). All clinical isolates were assigned an MS, whereas 188 of 206 (91.3%) were assigned a serotype by use of antisera. A small number of isolates (serosubtypes III-3 and III-4) showed different serotype specificities between PCR and sequencing, but the PCR results correlated with those obtained by CS. The overall agreement between the MS identification method and CS for isolates for which results of both tests were available was 100% (188 of 188 isolates). The MS identification method is a specific and practical alternative to conventional GBS serotyping and will facilitate epidemiological studies.
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