ArticleJournal of clinical microbiology2002
Comparison of six PCR methods using peripheral blood for detection of canine visceral leishmaniasis.
Article in Journal of clinical microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 100 papers, 1 of them a synthesis that pooled it.
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Who cites it
100 citing papers in PubMed, 1 synthesis or guideline pooled it, 392 citations in OpenAlex.
- Visceral leishmaniasis in leukemia patients, a great neglected disease in endemic countries: report of a retrospective study and systematic literature review.BMC infectious diseases · 2026Pooled it
- Looking Beyond Leishmania infantum: Serological and Molecular Investigation of Leishmania spp. in Domestic Dogs and Cats from a Visceral Leishmaniasis-Endemic Area in Northeastern Brazil.Acta parasitologica · 2026Article
- Validation of a duplex real-time PCR (qPCR) in clinically classified dogs naturally infected with Leishmania infantum before and after specific treatment: preliminary results.Parasites & vectors · 2026Article
- Three Leishmania species identified in domestic dogs in Tunisia: New insights into canine leishmaniosis.PLoS neglected tropical diseases · 2026Article
- Assessment of Leishmania exposure in blood donors from a non-endemic urban area: A study in São Paulo.Vox sanguinis · 2026Article
- TIM, a novel molecular toolbox for the detection and identification ofMicrobiology spectrum · 2026Article
- Presence or Emergence of Canine Leishmaniasis, Malawi.Emerging infectious diseases · 2026Article
- Possible role of domestic cats in the epidemiology of leishmaniasis: evidence of genital lesions and vertical transmission.Veterinary research communications · 2025Article
- First report of visceral Leishmaniasis in abandoned dogs of the urban area in the central region of Colombia.Veterinary research communications · 2025Article
- Development and Validation of In-House Conventional and Multiplex PCR Methods for the Detection and Identification of Lophomonas spp.: An Innovative Approach.Journal of clinical laboratory analysis · 2025Article
- Spatial prediction of canine visceral leishmaniasis in an endemic urban area of Brazil.PloS one · 2025Article
- New immunomodulatory treatment protocol for canine leishmaniosis reduces parasitemia and proteinuria.PLoS neglected tropical diseases · 2024Article
- Veterinary perspectives on the urbanization of leishmaniosis in Morocco.Parasites & vectors · 2024Article
- A compared histopathological study on kidneys and eye bulbs in distinct clinical presentations of canine leishmaniasis by Leishmania infantum.Veterinary research communications · 2024Article
- Comparison of SensPERT®Veterinary world · 2024Article
- Asymptomatic Leishmania infantum infection in dogs and dog owners in an endemic area in southeast France.Parasite (Paris, France) · 2024Article
- Hematological and biochemical parameters correlated to hemorheology in Canine Monocytic Ehrlichiosis.Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria · 2024Article
- Absence of Leishmania spp. DNA in road-killed wild mammals in Southern Brazil.Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria · 2024Article
- Occurrence ofPathogens (Basel, Switzerland) · 2023Article
- Serological and Molecular Survey ofMicroorganisms · 2022Article
40 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The objectives of this study were to compare the sensitivities and reliabilities of different PCR methods for the diagnosis and epidemiological study of canine visceral leishmaniasis (CVL) using dog blood. We chose to work with peripheral blood, as this type of sampling is noninvasive, straightforward, and easy to repeat. Six PCR methods were compared: three primer pairs target genomic DNA, and the other three target kinetoplast (mitochondrial) DNA. Sensitivity, specificity, reproducibility, and ease of interpretation without hybridization were evaluated for each method. The assessment was first performed using artificial samples. All methods could detect less than one parasite per reaction tube. However, the sensitivities varied among the different methods by a factor of 500 on purified cultivated parasites and by a factor of 10,000 on seeded dog blood samples (i.e., from 10 to 10(-3) parasite per ml of blood for the latter). Only four methods were found sufficiently reliable for the diagnosis of CVL. They were tested on 37 dogs living in an area of endemicity and grouped according to clinical status and specific serology. Only the two methods targeting kinetoplast DNA (K13A-K13B and RV1-RV2) could detect the parasite in 100% of symptomatic infected dogs. Similarly, all seropositive dogs were found PCR positive by these methods versus 62% by the genomic-DNA-based methods. Finally, these kinetoplast-based methods proved clearly superior to the others in the detection of Leishmania in asymptomatic dogs. Our data allow the discussion of the advantages and drawbacks of highly sensitive versus moderately sensitive PCR methods in diagnosis and prevalence studies of CVL.
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