ArticleJournal of clinical microbiology2002
Evaluation of a novel heminested PCR assay based on the phosphoglucosamine mutase gene for detection of Helicobacter pylori in saliva and dental plaque.
Article in Journal of clinical microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 43 citations in OpenAlex.
- Oral Helicobacter pylori: a scoping review and pilot study on the PCR-culture detection paradox.Clinical oral investigations · 2026Article
- A visual denaturation bubble-mediated strand exchange amplification and RGB visual analysis-based assay for quantitative detection of Helicobacter pylori in saliva.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2023Article
- The validity evaluation of different 16srRNA gene primers for helicobacter detection urgently requesting to design new specific primers.Scientific reports · 2022Article
- Oral Microbiota, a Potential Determinant for the Treatment Efficacy of GastricPolish journal of microbiology · 2022Article
- Periodontitis and Helicobacter pylori Infection: Eradication and Periodontal Therapy Combination.European journal of dentistry · 2022Article
- Declining prevalence ofHeliyon · 2020Article
- GastricPeerJ · 2019Article
- Are the view of Helicobacter pylori colonized in the oral cavity an illusion?Experimental & molecular medicine · 2017Review
- Occurrence ofGastroenterology and hepatology from bed to bench · 2017Article
- Role of dental plaque, saliva and periodontal disease in Helicobacter pylori infection.World journal of gastroenterology · 2014Review
- Oral Cavity as an Extragastric Reservoir of Helicobacter pylori.ISRN gastroenterology · 2014Review
- Detection of helicobacter pylori in benign laryngeal lesions by polymerase chain reaction: a cross sectional study.Infectious agents and cancer · 2012Article
- Simultaneous amplification of two bacterial genes: more reliable method of Helicobacter pylori detection in microbial rich dental plaque samples.Current microbiology · 2011Article
- Helicobacter pylori infection in children.Saudi journal of gastroenterology : official journal of the Saudi Gastroenterology Association · 2009Article
- Unreliability of results of PCR detection of Helicobacter pylori in clinical or environmental samples.Journal of clinical microbiology · 2009Article
- Horizontal versus familial transmission of Helicobacter pylori.PLoS pathogens · 2008Article
- A population genetics pedigree perspective on the transmission of Helicobacter pylori.Genetics · 2006Article
- Incidence of Helicobacter felis and the effect of coinfection with Helicobacter pylori on the gastric mucosa in the African population.Journal of clinical microbiology · 2006Article
- Absence of Helicobacter pylori within the oral cavities of members of a healthy South African community.Journal of clinical microbiology · 2006Article
- Real-time quantitative PCR assay for the detection of Helicobacter pylori: no association with sudden infant death syndrome.International journal of legal medicine · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A novel heminested PCR protocol was developed for the specific detection of Helicobacter pylori at low copy numbers. A set of primers specific for the phosphoglucosamine mutase gene (glmM) of H. pylori produced a 765-bp fragment that was used as template for the heminested primer pair delineating a 496-bp fragment. By using agarose gel electrophoresis for detection of the heminested PCR-amplified products, amplification of H. pylori genomic DNA was achieved at concentrations as low as 0.1 pg, equivalent to 5 x 10(2) bacteria. A study was subsequently undertaken to evaluate the heminested PCR for detection of H. pylori in dental plaque and saliva. Specimens collected from 58 individuals were cultured, and PCR was subsequently performed on the oral cultures. Identification of H. pylori in the same series of saliva and dental plaque specimens was carried out with PCR using a primer pair specific for the H. pylori urease B gene and by the heminested PCR assay. The identity of the amplified products was confirmed by DNA sequencing. Our results demonstrate that the heminested PCR assay was specific for detection of H. pylori, yielding no false-positive results, and that H. pylori had a low prevalence (approximately 3%) in specimens obtained from the oral cavity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.