ArticleJournal of clinical microbiology2002
Molecular fingerprinting of Clostridium difficile isolates: pulsed-field gel electrophoresis versus amplified fragment length polymorphism.
Article in Journal of clinical microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 91 citations in OpenAlex.
- Genetic relatedness between pneumococcal populations originating from the nasopharynx, adenoid, and tympanic cavity of children with otitis media.Journal of clinical microbiology · 2005Trial
- Epidemiological and genomic characterization of community-acquired Clostridium difficile infections.BMC infectious diseases · 2018Article
- Advantages and limitations of potential methods for the analysis of bacteria in milk: a review.Journal of food science and technology · 2016Review
- Molecular epidemiology of Clostridium difficile strains from nosocomial-acquired infections.Folia microbiologica · 2014Article
- Molecular and microbiological characterization of Clostridium difficile isolates from single, relapse, and reinfection cases.Journal of clinical microbiology · 2012Article
- Extended multilocus variable-number tandem-repeat analysis of Clostridium difficile correlates exactly with ribotyping and enables identification of hospital transmission.Journal of clinical microbiology · 2011Article
- Comparison of strain typing results for Clostridium difficile isolates from North America.Journal of clinical microbiology · 2011Article
- Antimicrobial susceptibilities and molecular epidemiology of clinical isolates of Clostridium difficile in taiwan.Antimicrobial agents and chemotherapy · 2011Article
- Clostridium difficile bacteremia, Taiwan.Emerging infectious diseases · 2010Review
- Pseudo-outbreak of Clostridium sordellii infection following probable cross-contamination in a hospital clinical microbiology laboratory.Infection control and hospital epidemiology · 2010Article
- Rapid molecular characterization of Clostridium difficile and assessment of populations of C. difficile in stool specimens.Journal of clinical microbiology · 2009Article
- Microarray identification of Clostridium difficile core components and divergent regions associated with host origin.Journal of bacteriology · 2009Article
- The Exiguobacterium genus: biodiversity and biogeography.Extremophiles : life under extreme conditions · 2009Article
- A prospective study of risk factors and historical trends in metronidazole failure for Clostridium difficile infection.Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association · 2008Article
- Genotypic investigation of Clostridium difficile in Prince Edward Island.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2008Article
- Characterization of Clostridium difficile strains isolated from patients in Ontario, Canada, from 2004 to 2006.Journal of clinical microbiology · 2008Article
- Toxinotype V Clostridium difficile in humans and food animals.Emerging infectious diseases · 2008Article
- Molecular typing of a suspected cluster of Nocardia farcinica infections by use of randomly amplified polymorphic DNA, pulsed-field gel electrophoresis, and amplified fragment length polymorphism analyses.Journal of clinical microbiology · 2007Article
- Molecular analysis of Clostridium difficile at a university teaching hospital in Japan: a shift in the predominant type over a five-year period.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2007Article
- Differentiation of Bacillus anthracis, B. cereus, and B. thuringiensis by using pulsed-field gel electrophoresis.Applied and environmental microbiology · 2007Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two molecular fingerprinting techniques, pulsed-field gel electrophoresis (PFGE) and amplified fragment length polymorphism (AFLP), were used to investigate the epidemiological relatedness among Clostridium difficile isolates from suspected outbreaks in three general hospitals. Analysis by PFGE yielded inconclusive data as a result of extensive DNA degradation. Although this degradation could be prevented to a certain extent by the inclusion of thiourea in the electrophoresis buffer, the weak DNA banding patterns obtained in this way were still far from optimal. AFLP data were obtained by using fluorescently labeled PCR primers and analysis on an ABI PRISM automated DNA analysis platform. AFLP analysis yielded high resolution and highly reproducible DNA fingerprinting patterns from which the epidemiological relatedness among the isolates could easily be determined. AFLP results could be readily obtained within 24 h, whereas 3 to 4 days were routinely required to complete the lengthy PFGE protocol. AFLP clearly proved to be a much more fail-safe fingerprinting method for C. difficile isolates, especially for those isolates for which a standard PFGE procedure yielded inconclusive results due to DNA degradation.
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