ArticleThe Journal of biological chemistry2002
A human RNA polymerase II-containing complex associated with factors necessary for spliceosome assembly.
Article in The Journal of biological chemistry, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 54 citations in OpenAlex.
- U1 snRNP and RNA polymerase II interaction is predominantly mediated by Prp40 rather than U1-70K in yeast.Nucleic acids research · 2026Article
- Protein-interaction network analysis reveals the role of Prp19 splicing factor in transcription of both intron-containing and intron-lacking genes.PLoS genetics · 2026Article
- Pre-mRNA splicing and its cotranscriptional connections.Trends in genetics : TIG · 2023Review
- U2AFEMBO reports · 2019Article
- Chromatin changes in SMARCAL1 deficiency: A hypothesis for the gene expression alterations of Schimke immuno-osseous dysplasia.Nucleus (Austin, Tex.) · 2016Article
- Nuclear import of RNA polymerase II is coupled with nucleocytoplasmic shuttling of the RNA polymerase II-associated protein 2.Nucleic acids research · 2013Article
- Cotranscriptional splicing efficiency differs dramatically between Drosophila and mouse.RNA (New York, N.Y.) · 2012Article
- Co-transcriptional regulation of alternative pre-mRNA splicing.Biochimica et biophysica acta · 2012Review
- Penetrance of biallelic SMARCAL1 mutations is associated with environmental and genetic disturbances of gene expression.Human molecular genetics · 2012Article
- RNA-mediated displacement of an inhibitory snRNP complex activates transcription elongation.Nature structural & molecular biology · 2010Article
- Polyadenylation releases mRNA from RNA polymerase II in a process that is licensed by splicing.RNA (New York, N.Y.) · 2009Article
- Targeting tat inhibitors in the assembly of human immunodeficiency virus type 1 transcription complexes.Journal of virology · 2008Article
- Cotranscriptional splicing potentiates the mRNA production from a subset of estradiol-stimulated genes.Molecular and cellular biology · 2008Article
- A RECQ5-RNA polymerase II association identified by targeted proteomic analysis of human chromatin.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Proximity-dependent and proximity-independent trans-splicing in mammalian cells.RNA (New York, N.Y.) · 2008Article
- Functional coupling of last-intron splicing and 3'-end processing to transcription in vitro: the poly(A) signal couples to splicing before committing to cleavage.Molecular and cellular biology · 2008Article
- Functional coupling of RNAP II transcription to spliceosome assembly.Genes & development · 2006Article
- Role for PSF in mediating transcriptional activator-dependent stimulation of pre-mRNA processing in vivo.Molecular and cellular biology · 2005Article
- A subset of nuclear receptor coregulators act as coupling proteins during synthesis and maturation of RNA transcripts.Molecular and cellular biology · 2005Review
- The highly conserved glutamic acid 791 of Rpb2 is involved in the binding of NTP and Mg(B) in the active center of human RNA polymerase II.Nucleic acids research · 2005Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Transcription and splicing are coordinated processes in mammalian cells. We have used affinity chromatography with immobilized transcription elongation factor SII to purify a protein complex that contains core RNA polymerase II (RNA Pol II), the general transcription initiation factors, and several splicing factors, including the U1, U2, and U4 small nuclear RNPs, the U2AF(65), and serine/arginine-rich proteins. The splicing factors and the transcription machinery co-purify through a gel filtration column and co-immunoprecipitate in experiments using an anti-U2AF(65) antibody, indicating that they are part of a unique complex. Although the RNA Pol II-containing complex does not possess splicing activity, it can complement small nuclear RNP-inactivated extracts and can promote the formation of a pre-spliceosome complex. Because interactions between components of the splicing and transcription machineries occur in the context of a complex containing a hypophosphorylated RNA Pol II capable of initiating transcription, our results suggest that the coupling between transcription and splicing begins before transcription initiation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.