ArticleGut2002
Endothelin-1 stimulates human colonic myofibroblast contraction and migration.
Article in Gut, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 53 citations in OpenAlex.
- Cellular crosstalk of fibroblast-myofibroblast transition in intestinal homeostasis and disease.Biomarker research · 2026Review
- Biomechanical Force and Cellular Stiffness in Lung Fibrosis.The American journal of pathology · 2022Review
- Endothelin-1 axes in the framework of predictive, preventive and personalised (3P) medicine.The EPMA journal · 2021Review
- The NaCellular and molecular life sciences : CMLS · 2021Review
- Vascular endothelial growth factor-D mediates fibrogenic response in myofibroblasts.Molecular and cellular biochemistry · 2016Article
- Association of endothelin-1 and matrix metallopeptidase-9 with metabolic syndrome in middle-aged and older adults.Diabetology & metabolic syndrome · 2015Article
- Inducible NOS mediates CNP-induced relaxation of intestinal myofibroblasts.American journal of physiology. Gastrointestinal and liver physiology · 2013Article
- Cellular and molecular mechanisms of intestinal fibrosis.World journal of gastroenterology · 2012Article
- Thrombin-induced endothelin-1 synthesis and secretion in retinal pigment epithelial cells is rho kinase dependent.Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics · 2010Article
- Role of TGF-beta in proliferative vitreoretinal diseases and ROCK as a therapeutic target.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Inhibition of endothelin receptors in the treatment of pulmonary arterial hypertension: does selectivity matter?European heart journal · 2008Review
- In vitro wounding: effects of hypoxia and transforming growth factor beta1 on proliferation, migration and myofibroblastic differentiation in an endothelial cell-fibroblast co-culture model.Journal of molecular histology · 2008Article
- Endothelin-1 induces alveolar epithelial-mesenchymal transition through endothelin type A receptor-mediated production of TGF-beta1.American journal of respiratory cell and molecular biology · 2007Article
- Endothelin-1 stimulates contraction and migration of rat pancreatic stellate cells.World journal of gastroenterology · 2005Article
- Effects of angiotensin II receptor signaling during skin wound healing.The American journal of pathology · 2004Article
- Endothelin-1: a multifunctional molecule in cancer.British journal of cancer · 2003Review
- Single-cell epithelial defects close rapidly by an actinomyosin purse string mechanism with functional tight junctions.The Journal of physiology · 2002Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundAlthough the contractile, migratory, and proliferative responses of subepithelial myofibroblasts to injury have been postulated to be important events in intestinal wound healing, contractile force generation and migration by these cells has not been investigated previously, and the signals that regulate proliferation by these cells are poorly understood.
aimsThe primary aim of this study was to test the hypothesis that the inflammatory mediator endothelin-1 modulates contraction, migration, and proliferation of intestinal myofibroblasts. We also sought to examine the signal transduction pathways which might underlie these putative effects.
methodsContraction, migration, proliferation, cytosolic [Ca(2+)], and myosin phosphorylation were measured in human colonic subepithelial myofibroblasts in the absence and presence of endothelin receptor agonists and antagonists.
resultsEndothelin-1, but not interleukin 1 alpha, interleukin 6, interleukin 8, interleukin 10, or tumour necrosis factor alpha, induced a rapid and robust generation of contractile force, which was associated with an increase in cytosolic [Ca(2+)] and myosin phosphorylation. Inhibition of rho associated kinase reduced endothelin-1 stimulated myosin phosphorylation and contractile force development. Endothelin-1 stimulated migration with a dose-response relationship similar to that observed for contraction. Endothelin A and B receptors mediated contraction while migration was mediated predominantly through endothelin B receptors. Platelet derived growth factor and serum, but not endothelin-1, induced proliferation.
conclusionsEndothelin-1 stimulates colonic subepithelial myofibroblast contraction and migration via endothelin receptor mediated myosin phosphorylation. These results support an important role for subepithelial myofibroblasts in the injury response of the gut and consequently intestinal wound repair.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.