ArticleApplied and environmental microbiology2002
Quantification of Nitrosomonas oligotropha-like ammonia-oxidizing bacteria and Nitrospira spp. from full-scale wastewater treatment plants by competitive PCR.
Article in Applied and environmental microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
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67 citing papers in PubMed, 428 citations in OpenAlex.
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- Nutrient removal performance and microbial composition analysis in hybrid membrane bioreactor for municipal wastewater treatment.Bioprocess and biosystems engineering · 2025Article
- Wastewater treatment from a science faculty during the COVID-19 pandemic by using ammonium-oxidising and heterotrophic bacteria.3 Biotech · 2024Article
- Microbial, chemical, and isotopic monitoring integrated approach to assess potential leachate contamination of groundwater in a karstic aquifer (Apulia, Italy).Environmental monitoring and assessment · 2024Article
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- The Dark Side of Microbial Processes: Accumulation of Nitrate During Storage of Surface Water in the Dark and the Underlying Mechanism.Microbiology spectrum · 2022Article
- Partition of Anammox and Nitrifiers Through Bio-Carriers for Full-Scale Sidestream Partial Nitrification-Anammox Plant.Frontiers in bioengineering and biotechnology · 2022Article
- Endpoint Recombinase Polymerase Amplification (RPA) Assay for Enumeration of Thiocyanate-degrading Bacteria.Microbes and environments · 2022Article
- The Dynamic Response of Nitrogen Transformation to the Dissolved Oxygen Variations in the Simulated Biofilm Reactor.International journal of environmental research and public health · 2021Article
- Lost in translation: the quest for Nitrosomonas cluster 7-specific amoA primers and TaqMan probes.Microbial biotechnology · 2020Article
- Deep amoA amplicon sequencing reveals community partitioning within ammonia-oxidizing bacteria in the environmentally dynamic estuary of the River Elbe.Scientific reports · 2020Article
- Enhanced nitrogen removal in filled-and-drained vertical flow constructed wetlands: microbial responses to aeration mode and carbon source.Environmental science and pollution research international · 2020Article
- Intensified simultaneous nitrification and denitrification performance in integrated packed bed bioreactors using PHBV with different dosing methods.Environmental science and pollution research international · 2020Article
- Microbial community profiling of ammonia and nitrite oxidizing bacterial enrichments from brackishwater ecosystems for mitigating nitrogen species.Scientific reports · 2020Article
- Density-Based Separation of Microbial Functional Groups in Activated Sludge.International journal of environmental research and public health · 2020Article
- Autotrophic Fixed-Film Systems Treating High Strength Ammonia Wastewater.Frontiers in microbiology · 2020Article
- Nitrogen Fertilizer Amendment Alter the Bacterial Community Structure in the Rhizosphere of Rice (Frontiers in microbiology · 2019Article
- Putative Mixotrophic Nitrifying-Denitrifying Gammaproteobacteria Implicated in Nitrogen Cycling Within the Ammonia/Oxygen Transition Zone of an Oil Sands Pit Lake.Frontiers in microbiology · 2019Article
- Stochastic processes govern invasion success in microbial communities when the invader is phylogenetically close to resident bacteria.The ISME journal · 2018Article
7 more citing papers are in PubMed but not listed here.
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6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Utilizing the principle of competitive PCR, we developed two assays to enumerate Nitrosomonas oligotropha-like ammonia-oxidizing bacteria and nitrite-oxidizing bacteria belonging to the genus NITROSPIRA: The specificities of two primer sets, which were designed for two target regions, the amoA gene and Nitrospira 16S ribosomal DNA (rDNA), were verified by DNA sequencing. Both assays were optimized and applied to full-scale, activated sludge wastewater treatment plant (WWTP) samples. If it was assumed that there was an average of 3.6 copies of 16S rDNA per cell in the total population and two copies of the amoA gene per ammonia-oxidizing bacterial cell, the ammonia oxidizers examined represented 0.0033% +/- 0.0022% of the total bacterial population in a municipal WWTP. N. oligotropha-like ammonia-oxidizing bacteria were not detected in an industrial WWTP. If it was assumed that there was one copy of the 16S rDNA gene per nitrite-oxidizing bacterial cell, Nitrospira spp. represented 0.39% +/- 0.28% of the biosludge population in the municipal WWTP and 0.37% +/- 0.23% of the population in the industrial WWTP. The number of Nitrospira sp. cells in the municipal WWTP was more than 62 times greater than the number of N. oligotropha-like cells, based on a competitive PCR analysis. The results of this study extended our knowledge of the comparative compositions of nitrifying bacterial populations in wastewater treatment systems. Importantly, they also demonstrated that we were able to quantify these populations, which ultimately will be required for accurate prediction of process performance and stability for cost-effective design and operation of WWTPs.
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