ArticleApplied and environmental microbiology2002
Role of the single regulator MrsR1 and the two-component system MrsR2/K2 in the regulation of mersacidin production and immunity.
Article in Applied and environmental microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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27 citing papers in PubMed, 63 citations in OpenAlex.
- The DdD protein confers intracellular and extracellular immunity to the leaderless bacteriocin enterocin DD14.PNAS nexus · 2026Article
- Phylogenetic classification of natural product biosynthetic gene clusters based on regulatory mechanisms.Frontiers in microbiology · 2023Article
- Modular Use of the Uniquely Small Ring A of Mersacidin Generates the Smallest Ribosomally Produced Lanthipeptide.ACS synthetic biology · 2022Article
- Identification of a Novel Two-Peptide Lantibiotic from Vagococcus fluvialis.Microbiology spectrum · 2022Article
- Heterologous Expression of Mersacidin inACS synthetic biology · 2021Article
- Simultaneous Production of Multiple Antimicrobial Compounds byFrontiers in microbiology · 2021Article
- Bacteriocins, Antimicrobial Peptides from Bacterial Origin: Overview of Their Biology and Their Impact against Multidrug-Resistant Bacteria.Microorganisms · 2020Review
- CerR, a Single-Domain Regulatory Protein of the LuxR Family, Promotes Cerecidin Production and Immunity in Bacillus cereus.Applied and environmental microbiology · 2018Article
- Identification and classification of known and putative antimicrobial compounds produced by a wide variety of Bacillales species.BMC genomics · 2016Article
- Structure of the Response Regulator NsrR from Streptococcus agalactiae, Which Is Involved in Lantibiotic Resistance.PloS one · 2016Article
- Purification and partial characterization of a thermostable antimicrobial protein from Bacillus subtilis FB123.World journal of microbiology & biotechnology · 2015Article
- Investigation of antibacterial activity of Bacillus spp. isolated from the feces of Giant Panda and characterization of their antimicrobial gene distributions.World journal of microbiology & biotechnology · 2014Article
- Antimicrobial Peptide Resistance Mechanisms of Gram-Positive Bacteria.Antibiotics (Basel, Switzerland) · 2014Article
- Bacterial competition reveals differential regulation of the pks genes by Bacillus subtilis.Journal of bacteriology · 2014Article
- Characterization of amylolysin, a novel lantibiotic from Bacillus amyloliquefaciens GA1.PloS one · 2013Article
- Insights into Lantibiotic Immunity Provided by Bioengineering of LtnI.Antimicrobial agents and chemotherapy · 2012Article
- The spiFEG locus in Streptococcus infantarius subsp. infantarius BAA-102 confers protection against nisin U.Antimicrobial agents and chemotherapy · 2012Article
- Transcription analysis of a lantibiotic gene cluster from Bifidobacterium longum DJO10A.Applied and environmental microbiology · 2011Article
- Article
- Dissecting structural and functional diversity of the lantibiotic mersacidin.Chemistry & biology · 2009Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The lantibiotic mersacidin is an antimicrobial peptide of 20 amino acids which inhibits bacterial cell wall biosynthesis by binding to the precursor molecule lipid II and which is produced by Bacillus sp. strain HIL Y-85,54728. The structural gene of mersacidin as well as accessory genes is organized in a biosynthetic gene cluster which is located on the chromosome and contains three open reading frames with similarities to regulatory proteins: mrsR2 and mrsK2 encode two proteins with homology to bacterial two-component systems, and mrsR1 shows similarity to a response regulator. Both mrsR2/K2 and mrsR1 were inactivated by insertion of an antibiotic resistance marker. Disruption of mrsR1 resulted in loss of mersacidin production; however, producer self-protection was not impaired. In contrast, inactivation of mrsR2/K2 led to an increased susceptibility to mersacidin whereas biosynthesis of the lantibiotic remained unaffected. Binding of mersacidin to intact cells was significantly enhanced in the mrsR2/K2 knockout mutant. Reverse transcription-PCR analysis from total RNA preparations showed that in contrast to the wild-type strain, the structural genes of the ABC transporter MrsFGE were not transcribed in the knockout mutant. It was therefore concluded that producer self-protection against mersacidin is conferred by the ABC transporter MrsFGE and that the transcription of mrsFGE is regulated by MrsR2/K2, whereas production of the antibacterial peptide is solely activated by MrsR1.
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