ArticleApplied and environmental microbiology2002
Effect of cold starvation, acid stress, and nutrients on metabolic activity of Helicobacter pylori.
Article in Applied and environmental microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
48 citing papers in PubMed, 117 citations in OpenAlex.
- Cholesterol sulfate signaling facilitates persistent colonization of mucoid-coccoid H. pylori in gastric environment.International microbiology : the official journal of the Spanish Society for Microbiology · 2026Article
- Invasive guests: the role of non-oral bacteria in oral dysbiosis and systemic health.Journal of oral microbiology · 2026Review
- CoccoidVirulence · 2025Review
- Comparison of three methods for generating the coccoid form of Helicobacter pylori and proteomic analysis.BMC microbiology · 2024Article
- Origin of fecal contamination in lettuce and strawberries: From microbial indicators, molecular markers, andHeliyon · 2024Article
- Use of Deep-Amplicon Sequencing (DAS), Real-Time PCR and In Situ Hybridization to DetectDiagnostics (Basel, Switzerland) · 2024Article
- Review
- Interplay between Amoxicillin Resistance and Osmotic Stress in Helicobacter pylori.Journal of bacteriology · 2022Article
- An Anaerobic Environment Drives the Harboring ofBiology · 2022Article
- Temperatures Outside the Optimal Range forBiology · 2021Article
- Nutrient Deficiency Promotes the Entry ofBiology · 2021Article
- Role of amoebae for survival and recovery of 'non-culturable' Helicobacter pylori cells in aquatic environments.FEMS microbiology ecology · 2020Article
- Resuscitation of the Helicobacter pylori Coccoid Forms by Resuscitation Promoter Factor Obtained from Micrococcus Luteus.Current microbiology · 2020Article
- The Puzzle of Coccoid Forms ofAntibiotics (Basel, Switzerland) · 2020Review
- Transformation ofPathogens (Basel, Switzerland) · 2020Review
- In vitro antimicrobial activity of immobilised essential oil components against Helicobacter pylori.World journal of microbiology & biotechnology · 2019Article
- Comparative Proteomic and Morphological Change Analyses ofFrontiers in microbiology · 2018Article
- Morphology ofPrzeglad gastroenterologiczny · 2018Review
- Enhance nisin yield via improving acid-tolerant capability of Lactococcus lactis F44.Scientific reports · 2016Article
- Three Tests Used to Identify Non-Culturable Form of Helicobacter pylori in Water Samples.Jundishapur journal of microbiology · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Helicobacter pylori can transform, in vivo as well as in vitro, from dividing spiral-shaped forms into nonculturable coccoids, with intermediate forms called U forms. The importance of nonculturable coccoid forms of H. pylori in disease transmission and antibiotic treatment failures is unclear. Metabolic activities of actively growing as well as nonculturable H. pylori were investigated by comparing the concentrations of cellular ATP and total RNA, gene expression, presence of cytoplasmic polyphosphate granules and iron inclusions, and cellular morphology during extended broth culture and nutritional cold starvation. In addition, the effect of exposing broth-cultured or cold-starved cells to a nutrient-rich or acidic environment on the metabolic activities was investigated. ATP was detectable up to 14 days and for at least 25 days after transformation from the spiral form to the coccoid form or U form in broth-cultured and cold-starved cells, respectively. mRNAs of VacA, a 26-kDa protein, and urease A were detected by using reverse transcription-PCR in cells cultured for 2 months in broth or cold starved for at least 28 months. The ATP concentration was not affected during exposure to fresh or acidified broth, while 4- to 12-h exposures of nonculturable cells to lysed human erythrocytes increased cellular ATP 12- to 150-fold. Incubation of nonculturable cold-starved cells with an erythrocyte lysate increased total RNA expression and ureA mRNA transcription as measured by quantitative real-time reverse transcription-PCR. Furthermore, the number of structurally intact starved coccoids containing polyphosphate granules increased almost fourfold (P = 0.0022) under the same conditions. In conclusion, a specific environmental stimulus can induce ATP, polyphosphate, and RNA metabolism in nonculturable H. pylori, indicating viability of such morphological forms.
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Registered trials
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