ArticleThe Biochemical journal2002
Sarcolipin uncouples hydrolysis of ATP from accumulation of Ca2+ by the Ca2+-ATPase of skeletal-muscle sarcoplasmic reticulum.
Article in The Biochemical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
73 citing papers in PubMed, 1 synthesis or guideline pooled it, 135 citations in OpenAlex.
- Gut Microbial Community and Host Thermoregulation in Small Mammals.Frontiers in physiology · 2022Pooled it
- Effects of mild-exercise training cessation in human skeletal muscle.European journal of applied physiology · 2012Trial
- Guardians of T-Cell CaCells · 2026Review
- Sarcolipin-SERCA uncoupling as a plausible heat source for sustaining fever.Frontiers in physiology · 2026Article
- Identification of a molecular resistor that controls UCP1-independent CaCell metabolism · 2025Article
- Cellular Feimin enhances exercise performance by suppressing muscle thermogenesis.Nature metabolism · 2025Article
- Systemic inhibition ofbioRxiv : the preprint server for biology · 2024Article
- Spaceflight increases sarcoplasmic reticulum CaNPJ microgravity · 2024Article
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- The role of brown adipose tissue in mediating healthful longevity.The journal of cardiovascular aging · 2024Article
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- Reproductive Functions of the Mitochondrial Progesterone Receptor (PR-M).Reproductive sciences (Thousand Oaks, Calif.) · 2023Review
- Hypermetabolism in mice carrying a near complete human chromosome 21.bioRxiv : the preprint server for biology · 2023Article
- The multiple facets of mitochondrial regulations controlling cellular thermogenesis.Cellular and molecular life sciences : CMLS · 2022Review
- Sarco(endo)plasmic reticulum CaiScience · 2022Article
- The Role of Thermogenic Fat Tissue in Energy Consumption.Current issues in molecular biology · 2022Review
- Knockdown of sarcolipin (SLN) impairs substrate utilization in human skeletal muscle cells.Molecular biology reports · 2022Article
- Whole-body endothermy: ancient, homologous and widespread among the ancestors of mammals, birds and crocodylians.Biological reviews of the Cambridge Philosophical Society · 2022Article
- Metabolically driven maturation of human-induced-pluripotent-stem-cell-derived cardiac microtissues on microfluidic chips.Nature biomedical engineering · 2022Article
- CaProceedings of the National Academy of Sciences of the United States of America · 2022Article
13 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sarcolipin (SLN) is a small peptide found in the sarcoplasmic reticulum of skeletal muscle. It is predicted to contain a single hydrophobic transmembrane alpha-helix. Fluorescence emission spectra for the single Trp residue of SLN suggest that SLN incorporates fully into bilayers of dioleoylphosphatidylcholine, but only partially into bilayers of phosphatidylcholines with long (C(22) or C(24)) fatty acyl chains. The fluorescence of SLN is quenched in bilayers of dibromostearoylphosphatidylcholine, also consistent with incorporation into the lipid bilayer. SLN was reconstituted with the Ca(2+)-ATPase of skeletal-muscle sarcoplasmic reticulum. Even at a 50:1 molar ratio of SLN/ATPase, SLN had no significant effect on the rate of ATP hydrolysis by the ATPase or on the Ca(2+)-dependence of ATP hydrolysis. However, at a molar ratio of SLN/ATPase of 2:1 or higher the presence of SLN resulted in a marked decrease in the level of accumulation of Ca(2+) by reconstituted vesicles. The effect of SLN was structurally specific and did not result from a breakdown in the vesicular structure or from the formation of non-specific ion channels. Vesicles were impermeable to Ca(2+) in the absence of ATP in the external medium. The effects of SLN on accumulation of Ca(2+) can be simulated assuming that SLN increases the rate of slippage on the ATPase and the rate of passive leak of Ca(2+) mediated by the ATPase. It is suggested that the presence of SLN could be important in non-shivering thermogenesis, a process in which heat is generated by hydrolysis of ATP by skeletal-muscle sarcoplasmic reticulum.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.