ArticleJournal of muscle research and cell motility2001
Changes in mitochondrial Ca2+ detected with Rhod-2 in single frog and mouse skeletal muscle fibres during and after repeated tetanic contractions.
Article in Journal of muscle research and cell motility, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 47 citations in OpenAlex.
- Elevation of Mitochondrial CaCells · 2026Article
- How Priming Exercise Affects Oxygen Uptake Kinetics: From Underpinning Mechanisms to Endurance Performance.Sports medicine (Auckland, N.Z.) · 2023Review
- Mitochondrial Dysfunction in Intensive Care Unit-Acquired Weakness and Critical Illness Myopathy: A Narrative Review.International journal of molecular sciences · 2023Review
- A controversial issue: Can mitochondria modulate cytosolic calcium and contraction of skeletal muscle fibers?The Journal of general physiology · 2022Review
- Association of mitochondrial haplogroup F with physical performance in Korean population.Genomics & informatics · 2019Article
- Molecular Basis for Exercise-Induced Fatigue: The Importance of Strictly Controlled Cellular CaCold Spring Harbor perspectives in medicine · 2018Review
- The excitation-contraction coupling mechanism in skeletal muscle.Biophysical reviews · 2014Review
- Usage of a localised microflow device to show that mitochondrial networks are not extensive in skeletal muscle fibres.PloS one · 2014Article
- Mitochondrial calcium uptake.Proceedings of the National Academy of Sciences of the United States of America · 2013Review
- On-off asymmetries in oxygen consumption kinetics of single Xenopus laevis skeletal muscle fibres suggest higher-order control.The Journal of physiology · 2013Article
- Mitochondrial activation at the onset of contractions in isolated myofibres during successive contractile periods.The Journal of physiology · 2012Article
- Differential impact of mitochondrial positioning on mitochondrial Ca(2+) uptake and Ca(2+) spark suppression in skeletal muscle.American journal of physiology. Cell physiology · 2011Article
- Mitochondrial calcium uptake regulates rapid calcium transients in skeletal muscle during excitation-contraction (E-C) coupling.The Journal of biological chemistry · 2011Article
- Sarcoplasmic reticulum-mitochondrial through-space coupling in skeletal muscle.Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme · 2009Article
- Sarcoplasmic reticulum-mitochondrial symbiosis: bidirectional signaling in skeletal muscle.Exercise and sport sciences reviews · 2009Review
- The use of CalciumOrange-5N as a specific marker of mitochondrial Ca2+ in mouse skeletal muscle fibers.Pflugers Archiv : European journal of physiology · 2008Article
- Mice expressing L345P mutant desmin exhibit morphological and functional changes of skeletal and cardiac mitochondria.Journal of muscle research and cell motility · 2008Article
- Effect of mitochondria poisoning by FCCP on Ca2+ signaling in mouse skeletal muscle fibers.Pflugers Archiv : European journal of physiology · 2008Article
- Non-invasive in vivo imaging of calcium signaling in mice.PloS one · 2007Article
- Confocal imaging of [Ca2+] in cellular organelles by SEER, shifted excitation and emission ratioing of fluorescence.The Journal of physiology · 2005Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The present study investigated mitochondrial Ca2+ uptake and release in intact living skeletal muscle fibres subjected to bouts of repetitive activity. Confocal microscopy was used in conjunction with the Ca2+-sensitive dye Rhod-2 to monitor changes in mitochondrial Ca2+ in single Xenopus or mouse muscle fibres. A marked increase in the mitochondrial Ca2+ occurred in Xenopus fibres after 10 tetani applied at 4 s intervals. The mitochondrial Ca2+ continued to increase with increasing number of tetani. After the end of tetanic stimulation, mitochondrial Ca2+ declined to 50% of the maximal increase within 10 min and thereafter took up to 60 min to return to its original value. Depolarization of the mitochondria with FCCP greatly attenuated the rise in the mitochondrial Ca2+ evoked by repetitive tetanic stimulation. In addition, FCCP slowed the rate of decay of the tetanic Ca2+ transient which in turn led to an elevation of resting cytosolic Ca2+. Accumulation of Ca2+ in the mitochondria was accompanied by a modest mitochondrial depolarization. In contrast to the situation in Xenopus fibres, mitochondria in mouse toe muscle fibres did not show any change in the mitochondrial Ca2+ during repetitive stimulation and FCCP had no effect on the rate of decay of the tetanic Ca2+ transient. It is concluded that in Xenopus fibres, mitochondria play a role in the regulation of cytosolic Ca2+ and contribute to the relaxation of tetanic Ca2+ transients. In contrast to their important role in Xenopus fibres, mitochondria in mouse fast-twitch skeletal fibres play little role in Ca2+ homeostasis.
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