ArticlePharmaceutical research2001
Effect of probenecid on fluorescein transport in the central nervous system using in vitro and in vivo models.
Article in Pharmaceutical research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 51 citations in OpenAlex.
- Bile duct ligation-induced cirrhosis does not alter the blood-brain barrier permeability to sucrose in rats.Metabolic brain disease · 2024Article
- Brain-to-blood transport of fluorescein in vitro.Scientific reports · 2024Article
- Integrating Continuous Transepithelial Flux Measurements into an Ussing Chamber Set-Up.International journal of molecular sciences · 2024Article
- Probenecid, an Old Drug with Potential New Uses for Central Nervous System Disorders and Neuroinflammation.Biomedicines · 2023Review
- Factors Influencing Luciferase-Based Bioluminescent Imaging in Preclinical Models of Brain Tumor.Drug metabolism and disposition: the biological fate of chemicals · 2022Article
- Understanding the brain uptake and permeability of small molecules through the BBB: A technical overview.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2021Review
- Characterization of the blood-brain barrier in genetically diverse laboratory mouse strains.Fluids and barriers of the CNS · 2021Article
- LC-MS/MS-based in vitro and in vivo investigation of blood-brain barrier integrity by simultaneous quantitation of mannitol and sucrose.Fluids and barriers of the CNS · 2020Article
- Probenecid and N-Acetylcysteine Prevent Loss of Intracellular Glutathione and Inhibit Neuronal Death after Mechanical Stretch Injury In Vitro.Journal of neurotrauma · 2016Article
- Towards longitudinal mapping of extracellular pH in gliomas.NMR in biomedicine · 2016Article
- Adenosine receptor agonist NECA increases cerebral extravasation of fluorescein and low molecular weight dextran independent of blood-brain barrier modulation.Scientific reports · 2016Article
- Compromised blood-brain barrier permeability: novel mechanism by which circulating angiotensin II signals to sympathoexcitatory centres during hypertension.The Journal of physiology · 2016Review
- PET and SPECT radiotracers to assess function and expression of ABC transporters in vivo.Current drug metabolism · 2011Review
- Uptake, distribution and diffusivity of reactive fluorophores in cells: implications toward target identification.Molecular pharmaceutics · 2010Article
- P-glycoprotein deficient mouse in situ blood-brain barrier permeability and its prediction using an in combo PAMPA model.European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences · 2009Article
- Neuroinflammation facilitates LIF entry into brain: role of TNF.American journal of physiology. Cell physiology · 2008Article
- Decreased blood-brain barrier permeability to fluorescein in streptozotocin-treated rats.Neuroscience letters · 2007Article
- Mrp4 confers resistance to topotecan and protects the brain from chemotherapy.Molecular and cellular biology · 2004Article
- Cell handling, membrane-binding properties, and membrane-penetration modeling approaches of pivampicillin and phthalimidomethylampicillin, two basic esters of ampicillin, in comparison with chloroquine and azithromycin.Pharmaceutical research · 2003Article
- Impact of Mrp2 on the biliary excretion and intestinal absorption of furosemide, probenecid, and methotrexate using Eisai hyperbilirubinemic rats.Pharmaceutical research · 2003Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
purposeThe purpose of this study was to characterize the function of multidrug resistance-associated proteins (MRPs) (or MRP-like organic anion transport systems) in the blood-brain harrier (BBB) and blood-cerebrospinal fluid barrier (BCSFB) using both an in vitro BBB model and an in vivo microdialysis model.
methodsIn vitro functional studies were performed using bovine brain microvessel endothelial cells (BBMEC). The accumulation of fluorescein, an anionic fluorescent dye, in BBMEC was determined with and without the presence of inhibitors of various efflux transport proteins. In vivo microdialysis simultaneously monitored fluorescein concentrations in cortical extracellular fluid and cerebrospinal fluid. The effect of probenecid on the in vivo distribution of fluorescein was studied using a balanced crossover design in the rat.
resultsIn vitro experiments showed that probenecid, indomethacin, LY-329146, and all MRP inhibitors significantly increased (two- to threefold) the accumulation of fluorescein in BBMEC, whereas LY-335979, a P-gp inhibitor, had no effect on the accumulation of fluorescein. Probenecid significantly increased fluorescein plasma concentration and the plasma free fraction in vivo. The distribution of fluorescein across the BBB and BCSFB was enhanced by 2.2- and 1.9-fold, respectively, when probenecid was coadministered, even after correction for increased fluorescein plasma concentrations and free fraction.
conclusionsThese results demonstrate that MRPs or MRP-like transport system(s) may play an important role in fluorescein distribution across both BBB and BCSFB. This study showed that microdialysis proved to be a powerful in vivo technique for the study of transport systems in the central nervous system, and in vitro/in vivo correlations are possible using these model systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.