ArticleMolecular and cellular biology2002
Transcriptional interference by independently regulated genes occurs in any relative arrangement of the genes and is influenced by chromosomal integration position.
Article in Molecular and cellular biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 86 papers.
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Who cites it
86 citing papers in PubMed.
- Article
- Transcriptional interference revisited.Nature genetics · 2026Review
- Comparative analysis of convergent and divergent T7 RNA polymerase promoters for the synthesis of dsRNA in vivo and in vitro.RNA (New York, N.Y.) · 2025Article
- Defining gene ends: RNA polymerase II CTD threonine 4 phosphorylation marks transcription termination regions genome-wide.Nucleic acids research · 2025Article
- Optimizing effector functions of monoclonal antibodies via tailored N-glycan engineering using a dual landing pad CHO targeted integration platform.Scientific reports · 2023Article
- Mining theFrontiers in plant science · 2023Article
- Vector design for enhancing expression level and assembly of knob-into-hole based FabscFv-Fc bispecific antibodies in CHO cells.Antibody therapeutics · 2022Article
- SugarcaneInternational journal of molecular sciences · 2022Article
- A desirable transgenic strategy using GGTA1 endogenous promoter-mediated knock-in for xenotransplantation model.Scientific reports · 2022Article
- Article
- Recombinant antibodies aggregation and overcoming strategies in CHO cells.Applied microbiology and biotechnology · 2022Review
- Construction of PIK3C3 Transgenic Pig and Its Pathogenesis of Liver Damage.Life (Basel, Switzerland) · 2022Article
- DNA supercoiling-mediated collective behavior of co-transcribing RNA polymerases.Nucleic acids research · 2022Article
- Analytical kinetic model of native tandem promoters in E. coli.PLoS computational biology · 2022Article
- Review
- A Novel Cre/International journal of molecular sciences · 2021Article
- Control of Multigene Expression Stoichiometry in Mammalian Cells Using Synthetic Promoters.ACS synthetic biology · 2021Article
- Taming the Turmoil Within: New Insights on the Containment of Transposable Elements.Trends in genetics : TIG · 2020Review
- Full-length RNA profiling reveals pervasive bidirectional transcription terminators in bacteria.Nature microbiology · 2019Article
- Transcription termination sequences support the expression of transgene product secreted with milk.Transgenic research · 2019Article
26 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors.
Funding
Abstract
Transcriptional interference is the influence, generally suppressive, of one active transcriptional unit on another unit linked in cis. Its wide occurrence in experimental systems suggests that it may also influence transcription in many loci, but little is known about its precise nature or underlying mechanisms. Here we report a study of the interaction of two nearly identical transcription units juxtaposed in various arrangements. Each reporter gene in the constructs has its own promoter and enhancer and a strong polyadenylation signal. We used recombinase-mediated cassette exchange (RMCE) to insert the constructs into previously tagged genomic sites in cultured cells. This strategy also allows the constructs to be assessed in both orientations with respect to flanking chromatin. In each of the possible arrangements (tandem, divergent, and convergent), the presence of two genes strongly suppresses expression of both genes compared to that of an identical single gene at the same integration site. The suppression is most severe with the convergent arrangement and least severe in total with the divergent arrangement, while the tandem arrangement is most strongly influenced by the integration site and the genes' orientation within the site. These results suggest that transcriptional interference could underlie some position effects and contribute to the regulation of genes in complex loci.
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