ArticleThe Journal of cell biology2001
Autocrine epidermal growth factor signaling stimulates directionally persistent mammary epithelial cell migration.
Article in The Journal of cell biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 81 citations in OpenAlex.
- Immunomodulatory Effect of Epidermal Growth Factor Secreted by Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells on Atopic Dermatitis.International journal of stem cells · 2022Article
- Recellularization and Integration of Dense Extracellular Matrix by Percolation of Tissue Microparticles.Advanced functional materials · 2021Article
- Lysine-Specific Demethylase 1 Mediates AKT Activity and Promotes Epithelial-to-Mesenchymal Transition inMolecular cancer research : MCR · 2020Article
- Computational model of wound healing: EGF secreted by fibroblasts promotes delayed re-epithelialization of epithelial keratinocytes.Integrative biology : quantitative biosciences from nano to macro · 2018Article
- Cell-Cell Contact and Receptor Tyrosine Kinase Signaling.Cold Spring Harbor perspectives in biology · 2018Review
- Self-organization of chemoattractant waves in Dictyostelium depends on F-actin and cell-substrate adhesion.Journal of the Royal Society, Interface · 2016Article
- High-throughput protease activity cytometry reveals dose-dependent heterogeneity in PMA-mediated ADAM17 activation.Integrative biology : quantitative biosciences from nano to macro · 2015Article
- Navigator-3, a modulator of cell migration, may act as a suppressor of breast cancer progression.EMBO molecular medicine · 2015Article
- Epithelial TRPV1 signaling accelerates gingival epithelial cell proliferation.Journal of dental research · 2014Article
- Acute drop in blood monocyte count differentiates NEC from other causes of feeding intolerance.Journal of perinatology : official journal of the California Perinatal Association · 2014Article
- Gefitinib selectively inhibits tumor cell migration in EGFR-amplified human glioblastoma.Neuro-oncology · 2013Article
- A truncation allele in vascular endothelial growth factor c reveals distinct modes of signaling during lymphatic and vascular development.Development (Cambridge, England) · 2013Article
- Cooperative roles of SDF-1α and EGF gradients on tumor cell migration revealed by a robust 3D microfluidic model.PloS one · 2013Article
- Plasma rich in growth factors (PRGF-Endoret) stimulates tendon and synovial fibroblasts migration and improves the biological properties of hyaluronic acid.Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA · 2012Article
- Microfluidics for mammalian cell chemotaxis.Annals of biomedical engineering · 2012Review
- Differences in adhesion and protrusion properties correlate with differences in migration speed under EGF stimulation.BMC biophysics · 2012Article
- Network analysis of epidermal growth factor signaling using integrated genomic, proteomic and phosphorylation data.PloS one · 2012Article
- Short-lived, transitory cell-cell interactions foster migration-dependent aggregation.PloS one · 2012Article
- Transautocrine signaling by membrane neuregulins requires cell surface targeting, which is controlled by multiple domains.The Journal of biological chemistry · 2011Article
- The heparin-binding domain of HB-EGF mediates localization to sites of cell-cell contact and prevents HB-EGF proteolytic release.Journal of cell science · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 3 institutions in 1 country.
Funding
Abstract
Cell responses to soluble regulatory factors may be strongly influenced by the mode of presentation of the factor, as in matrix-bound versus diffusible modes. The possibly diverse effect of presenting a growth factor in autocrine as opposed to exogenous (or paracrine) mode is an especially important issue in cell biology. We demonstrate here that migration behavior of human mammary epithelial cells in response to stimulation by epidermal growth factor (EGF) is qualitatively different for EGF presented in exogenous (paracrine), autocrine, and intracrine modes. When EGF is added as an exogenous factor to the medium of cells that express EGF receptor (EGFR) but not EGF, cell migration speed increases while directional persistence decreases. When these EGFR-expressing cells are made to also express via retroviral transfection EGF in protease-cleaveable transmembrane form on the plasma membrane, migration speed similarly increases, but directional persistence increases as well. Addition of exogenous EGF to these cells abrogates their enhanced directional persistence, reducing their directionality to a level similar to wild-type cells. If the EGFR-expressing cells are instead transduced with a gene encoding EGF in a soluble form, migration speed and directional persistence were unaffected. Thus, autocrine presentation of EGF at the plasma membrane in a protease-cleavable form provides these cells with an enhanced ability to migrate persistently in a given direction, consistent with their increased capability for organizing into gland-like structures. In contrast, an exogenous/paracrine mode of EGF presentation generates a "scattering" response by the cells. These findings emphasize the functional importance of spatial restriction of EGFR signaling, and suggest critical implications for growth factor-based therapeutic treatments.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.