ArticleProceedings of the National Academy of Sciences of the United States of America2001
Oligomeric structure of virion-associated and soluble forms of the simian immunodeficiency virus envelope protein in the prefusion activated conformation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 49 citations in OpenAlex.
- Griffithsin Retains Anti-HIV-1 Potency with Changes in gp120 Glycosylation and Complements Broadly Neutralizing Antibodies PGT121 and PGT126.Antimicrobial agents and chemotherapy · 2019Article
- The Unusual Transmembrane Partition of the Hexameric Channel of the Hepatitis C Virus.Structure (London, England : 1993) · 2018Article
- Structural basis for the binding specificity of human Recepteur d'Origine Nantais (RON) receptor tyrosine kinase to macrophage-stimulating protein.The Journal of biological chemistry · 2014Article
- Eliciting neutralizing antibodies with gp120 outer domain constructs based on M-group consensus sequence.Virology · 2014Article
- Comparative Immunogenicity of Evolved V1V2-Deleted HIV-1 Envelope Glycoprotein Trimers.PloS one · 2013Article
- Retroviral env glycoprotein trafficking and incorporation into virions.Molecular biology international · 2012Article
- Newcastle disease virus expressing human immunodeficiency virus type 1 envelope glycoprotein induces strong mucosal and serum antibody responses in Guinea pigs.Journal of virology · 2011Article
- Density contrast sedimentation velocity for the determination of protein partial-specific volumes.PloS one · 2011Article
- Stabilized HIV-1 envelope glycoprotein trimers lacking the V1V2 domain, obtained by virus evolution.The Journal of biological chemistry · 2010Article
- Characterization of Lassa virus glycoprotein oligomerization and influence of cholesterol on virus replication.Journal of virology · 2010Article
- Optimization of human immunodeficiency virus type 1 envelope glycoproteins with V1/V2 deleted, using virus evolution.Journal of virology · 2009Article
- Cryoelectron tomography of HIV-1 envelope spikes: further evidence for tripod-like legs.PLoS pathogens · 2008Article
- Inhibition of HIV-1 entry by antibodies: potential viral and cellular targets.Journal of internal medicine · 2007Review
- Article
- Restraining the conformation of HIV-1 gp120 by removing a flexible loop.The EMBO journal · 2006Article
- Nature of nonfunctional envelope proteins on the surface of human immunodeficiency virus type 1.Journal of virology · 2006Article
- Kinetic rates of antibody binding correlate with neutralization sensitivity of variant simian immunodeficiency virus strains.Journal of virology · 2005Article
- Soluble mimetics of human immunodeficiency virus type 1 viral spikes produced by replacement of the native trimerization domain with a heterologous trimerization motif: characterization and ligand binding analysis.Journal of virology · 2005Article
- Retrovirus envelope protein complex structure in situ studied by cryo-electron tomography.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Oligomerization of the SARS-CoV S glycoprotein: dimerization of the N-terminus and trimerization of the ectodomain.Biochemical and biophysical research communications · 2004Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The envelope proteins (env) of simian immunodeficiency virus (SIV) and HIV type 1 assemble to form noncovalently associated oligomers in the endoplasmic reticulum. After cleavage in a Golgi compartment, oligomeric env complexes are transported to the surface of infected cells, where incorporation into budding virions can occur. Difficulties in obtaining adequate quantities of virions retaining env, as well as the unstable nature and hydrophobicity of the oligomer, may account for the absence of previous biophysical studies to determine the oligomeric valency of membrane-associated env. The aim of this study was to evaluate the oligomeric state of SIV env before membrane-fusion activation. Virion-associated env, obtained by crosslinking and detergent extraction, and non-crosslinked secreted env ectodomain (recombinant gp140) were purified by lentil-lectin chromatography and gel filtration as single predominant species. Sedimentation equilibrium-derived mass values for both forms of SIV env were close to those predicted for trimeric assemblies. Determination of the mass of individual molecules by scanning transmission electron microscopy confirmed that SIV virion-associated env and gp140 formed largely homogeneous populations of trimers. Furthermore, a triangular or tri-lobed morphology was clearly visualized in a subset of the trimers.
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